The Landscape and Potential Regulatory Mechanism of Lysine 2-Hydroxyisobutyrylation of Protein in End-Stage Renal Disease

Zheng, Fengping1,2; Xu, Huixuan1; Huang, Shaoying1; Zhang, Cantong1; Li, Shanshan1; Wang, Kang1; Dai, Weier3; Zhang, Xinzhou1; Tang, Donge2; Dai, Yong1,2

2021-09-01

10.1159/000518424

NEPHRON   影响因子和分区

1660-8151

2235-3186

Background: Acetylation has a vital role in the pathogenesis of end-stage renal disease (ESRD). Lysine 2-hydroxyisobutyrylation (K-hib) is a novel type of acetylation. In this study, we aimed to reveal the key features of K-hib in peripheral blood monocytes (PBMCs) of patients with ESRD. Method: We combined TMT labeling with LC-MS/MS analysis to compare K-hib modification of PBMCs between 20 ESRD patients and 20 healthy controls. The pan 2-hydroxyisobutyrylation antibody-based affinity enrichment method was used to reveal the features of K-hib, and the bioinformatics analysis was conducted to analyze the pathology of these K-hib-modified proteins. Result: Compared to healthy controls, we identified 440 upregulated proteins and 552 downregulated proteins in PBMCs of ESRD, among which 579 K-hib sites on 324 upregulated proteins and 287 K-hib sites on 188 downregulated proteins were identified. The site abundance, distribution, and function of the K-hib protein were further analyzed. The bioinformatics analysis revealed that the Rho/ROCK signaling pathway was highly enriched in ESRD, suggesting that it might contribute to renal fibrosis in ESRD patients. Conclusion: In this study, we found that K-hib-modified proteins correlated with the occurrence and progression of ESRD.

End-stage renal disease Lysine 2-hydroxyisobutyrylation Tandem mass tag labeling Liquid chromatography with tandem mass spectrometry Renal fibrosis

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81671596]

Urology & Nephrology

Urology & Nephrology

WOS:000694037400001

KARGER

CLINICAL MEDICINE

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, Shenzhen Peoples Hosp,Clin Med Coll 2,Dept Nephro, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, bDept Clin Med Res Ctr,Shenzhen Peoples Hosp,Clin, Shenzhen, Peoples R China
3.Univ Texas Austin, Coll Nat Sci, Austin, TX 78712 USA

Zheng, Fengping,Xu, Huixuan,Huang, Shaoying,et al. The Landscape and Potential Regulatory Mechanism of Lysine 2-Hydroxyisobutyrylation of Protein in End-Stage Renal Disease[J]. NEPHRON,2021.

Zheng, Fengping.,Xu, Huixuan.,Huang, Shaoying.,Zhang, Cantong.,Li, Shanshan.,...&Dai, Yong.(2021).The Landscape and Potential Regulatory Mechanism of Lysine 2-Hydroxyisobutyrylation of Protein in End-Stage Renal Disease.NEPHRON.

Zheng, Fengping,et al."The Landscape and Potential Regulatory Mechanism of Lysine 2-Hydroxyisobutyrylation of Protein in End-Stage Renal Disease".NEPHRON (2021).