Interleukin-10 Modulates the Metabolism and Osteogenesis of Human Dental Pulp Stem Cells

Yuan, Li; You, Hongxia; Qin, Nianhong; Zuo, Wenxin1,2

2021-09-01

10.1089/cell.2021.0044

Cellular Reprogramming   影响因子和分区

2152-4971

2152-4998

The osteogenic differentiation of mesenchymal stem cells (MSCs) is strongly related with the inflammatory microenvironment. The ability of osteogenic differentiation of MSCs is vital for the bone tissue engineering. Interleukin (IL)-10, a well-known anti-inflammatory factor, plays a key role in tissue repair. Dental pulp stem cells (DPSCs), with the advantage of convenience of extraction, are suitable for the bone tissue engineering. Therefore, it is meaning to explore the effects of IL-10 on the osteogenic differentiation of DPSCs. The proliferation activity of DPSCs were evaluated by MTS assay (CellTiter 96(R) Aqueous One Solution Cell Proliferation Assay [Promega]) and real-time polymerase chain reaction (RT-PCR). The osteogenic differentiation of DPSCs were determined by Alizarin Red staining, RT-PCR, and alkaline phosphatase activity test. The glucose metabolism was detected by Mito Stress test and glycolysis assay. IL-10 (10 or 20 nM) could enhance the osteogenic differentiation of DPSCs and promoted the metabolic switch from glycolysis to oxidative phosphorylation (OXPHOS), whereas IL-10 (5 and 50 nM) has no obvious effects on the osteogenic differentiation of DPSCs. The OXPHOS inhibitor restrained the promotion of osteogenic differentiation induced by IL-10. These findings show that IL-10 can promote the osteogenesis of DPSCs through the activation of OXPHOS, which provides a potential way for enhancing the osteogenic differentiation of DPSCs in bone tissue engineering.

IL-10 dental pulp stem cell osteogenesis glucose metabolism

SCI

英语

通讯

Natural Science Foundation of Shenzhen Science and Technology Innovation Commission[CYJ20190807145815129]

Cell Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity

Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Genetics & Heredity

WOS:000693320800001

MARY ANN LIEBERT, INC

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Dept Stomatol, Clin Med Coll 2, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China

Yuan, Li,You, Hongxia,Qin, Nianhong,et al. Interleukin-10 Modulates the Metabolism and Osteogenesis of Human Dental Pulp Stem Cells[J]. Cellular Reprogramming,2021.

Yuan, Li,You, Hongxia,Qin, Nianhong,&Zuo, Wenxin.(2021).Interleukin-10 Modulates the Metabolism and Osteogenesis of Human Dental Pulp Stem Cells.Cellular Reprogramming.

Yuan, Li,et al."Interleukin-10 Modulates the Metabolism and Osteogenesis of Human Dental Pulp Stem Cells".Cellular Reprogramming (2021).