A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation

Xu, Xinzhu1,2,3; Zhao, Kun1,2,3; Ren, Wei1; Wu, Zhaoyang1; Yu, Wentao4,5; Shao, Chendi4,5; Shi, Kebin4,5,6; Xi, Peng1,7,8

2021-09-01

10.1002/jemt.23922

MICROSCOPY RESEARCH AND TECHNIQUE   影响因子和分区

1059-910X

1097-0029

STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.

Bessel modulation detector saturation STED microscopy

SCI

英语

通讯

National Key Research and Development Program of China[2017YFC0110202] ; National Natural Science Foundation of China[31971376,61729501,62025501] ; Natural Science Foundation of Beijing Municipality[JQ18019] ; Shenzhen Science and Technology Program[KQTD20170810110913065]

Anatomy & Morphology ; Life Sciences & Biomedicine - Other Topics ; Microscopy

Anatomy & Morphology ; Biology ; Microscopy

WOS:000692830600001

WILEY

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Peking Univ, Coll Future Technol, Dept Biomed Engn, Beijing 100871, Peoples R China
2.Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA USA
3.Emory Univ, Atlanta, GA 30322 USA
4.Peking Univ, State Key Lab Mesoscop Phys, Beijing 100871, Peoples R China
5.Peking Univ, Sch Phys, Frontiers Sci Ctr Nano Optoelect, Beijing 100871, Peoples R China
6.Shanxi Univ, Collaborat Innovat Ctr Extreme Opt, Taiyuan, Peoples R China
7.Southern Univ Sci & Technol, Coll Engn, Dept Biomed Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Shenzhen, Guangdong, Peoples R China
8.Peking Univ, Natl Biomed Imaging Ctr, Beijing, Peoples R China

Xu, Xinzhu,Zhao, Kun,Ren, Wei,et al. A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation[J]. MICROSCOPY RESEARCH AND TECHNIQUE,2021.

Xu, Xinzhu.,Zhao, Kun.,Ren, Wei.,Wu, Zhaoyang.,Yu, Wentao.,...&Xi, Peng.(2021).A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation.MICROSCOPY RESEARCH AND TECHNIQUE.

Xu, Xinzhu,et al."A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation".MICROSCOPY RESEARCH AND TECHNIQUE (2021).