Expression and function of channelrhodopsin 2 in mouse outer hair cells

Chen, Fangyi1,2; Wu, Tao2; Wilson, Teresa2; Subhash, Hrebesh2; Omelchenko, Irina2; Bateschell, Michael2; Wang, Lingyan2; Brigande, John2; Jiang, Zhi-Gen2; Nuttall, Alfred2; Mohanty, SK; Thakor, NV

10.1117/12.2003114

2013

0277-786X

OPTOGENETICS: OPTICAL METHODS FOR CELLULAR CONTROL

8586

San Francisco, CA, United states

1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

SPIE-INT SOC OPTICAL ENGINEERING

Outer hair cell (OHC) is widely accepted as the origin of cochlear amplification, a mechanism that accounts for the extreme sensitivity of the mammalian hearing. The key process of cochlear amplification is the reverse transduction, where the OHC changes its length under electrical stimulation. In this study, we developed a method to modulate electro-mechanical transduction with an optogenetic approach based on channelrhodopsin 2 (ChR2), a direct light-activated non-selective cation channel (NSCC). We specifically expressed ChR2 in mouse cochlea OHCs through in uterus injection of adenovirus vector with ChR2 in fusion with the fluorescent marker tdTomato. We also transfected ChR2(H134R), a point mutant of ChR2, with plasmid to an auditory cell line (HEI-OC1). With whole cell recording, we found that blue light (470 nm) elicited a current with a reversal potential around zero in both mouse OHCs and HEI-OC1 cells and generated depolarization in both cell types.

Auditory Outer hair cell Optogenetics Channelrhodopsin Patch-Clamp Cochlear microphonic

第一 ; 通讯

英语

CPCI-S ; EI

Communication Disorders[R01DC005983] ; Communication Disorders[R01DC000141]

Genetics & Heredity ; Optics

Genetics & Heredity ; Optics

WOS:000322901400009

20132216373171

Audition ; Bacteriophages ; Cell culture ; Electrophysiology ; Mammals

Bioengineering and Biology:461

Web of Science

1.South Univ Sci & Technol China, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
2.Oregon Hlth & Sci Univ, Oregon Hearing Res Ctr, Portland, OR 97239 USA

Chen, Fangyi,Wu, Tao,Wilson, Teresa,et al. Expression and function of channelrhodopsin 2 in mouse outer hair cells[C]. 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA:SPIE-INT SOC OPTICAL ENGINEERING,2013.