| 题名 | Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors |
| 作者 | |
| 通讯作者 | Zhang, Xuming |
| 发表日期 | 2019-09-06
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| DOI | |
| 发表期刊 | |
| ISSN | 2041-1723
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| 卷号 | 10 |
| 摘要 | Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 mu mol g(-1) RuBisCO min(-1) from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI期刊
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| 学校署名 | 其他
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| 资助项目 | Doctoral cooperation fund of Shandong Academy of Sciences[2017BSHZ007]
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| WOS研究方向 | Science & Technology - Other Topics
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| WOS类目 | Multidisciplinary Sciences
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| WOS记录号 | WOS:000484599200001
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| 出版者 | |
| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：58
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/25207 |
| 专题 | 工学院_电子与电气工程系 |
| 作者单位 | 1.Hong Kong Polytech Univ, Dept Appl Phys, Hong Kong, Peoples R China 2.Univ Macau, State Key Lab Analog & Mixed Signal VLSI, Inst Microelect, Macau, Peoples R China 3.Qilu Univ Technol, Shandong Acad Sci, Shandong Prov Key Lab Microbial Engn, Dept Bioengn,State Key Lab Biobased Mat & Green P, Jinan, Shandong, Peoples R China 4.Hong Kong Polytech Univ, State Key Lab Chem Biol & Drug Discovery, Food Safety & Technol Res Ctr, Dept Appl Biol & Chem Technol, Hong Kong, Peoples R China 5.Hong Kong Polytech Univ, State Key Lab Chinese Med & Mol Pharmacol Incubat, Shenzhen Key Lab Food Biol Safety Control, Shenzhen Res Inst, Shenzhen, Peoples R China 6.Hong Kong Polytech Univ, Univ Res Facil Life Sci, Hong Kong, Peoples R China 7.Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen, Peoples R China 8.Univ Macau, Fac Sci & Technol, Macau, Peoples R China 9.Univ Macau, Fac Hlth Sci, Macau, Peoples R China 10.Sun Yat Sen Univ, Sch Elect & Informat Engn, Guangzhou, Guangdong, Peoples R China 11.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China 12.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Key Lab Spectral Imaging Technol, Xian, Shaanxi, Peoples R China 13.Wuhan Univ, Sch Phys & Technol, Wuhan, Hubei, Peoples R China 14.Taiyuan Univ Technol, Coll Informat & Comp Sci, MircoNano Syst Res Ctr, Taiyuan, Shanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Zhu, Yujiao,Huang, Ziyu,Chen, Qingming,et al. Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors[J]. Nature Communications,2019,10.
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| APA |
Zhu, Yujiao.,Huang, Ziyu.,Chen, Qingming.,Wu, Qian.,Huang, Xiaowen.,...&Zhang, Xuming.(2019).Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors.Nature Communications,10.
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| MLA |
Zhu, Yujiao,et al."Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors".Nature Communications 10(2019).
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