南方科技大学知识苑(SUSTech KC): A 54-kDa short variant of DHX33 functions in regulating mRNA translation

题名	A 54-kDa short variant of DHX33 functions in regulating mRNA translation
作者	Su, Dan; Yuan, Baolei; Su, Chenjing; Zhang, Yandong
通讯作者	Zhang, Yandong
发表日期	2019-09
DOI	10.1002/jcp.28178
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY 影响因子和分区
ISSN	0021-9541
EISSN	1097-4652
卷号	234 期号:9页码:15308-15319
摘要	DEAH box protein DHX33 has been found to be necessary for cell proliferation and early development of multicellular organisms. It plays diverse roles in regulating gene transcription, ribosome RNA synthesis, and protein translation. Dysregulation of DHX33 has been observed in various human cancers. In this study, we identified a short DHX33 variant in cells. The short DHX33 (hereafter referred to as DHX33-2) has only 534 amino acids, which completely matches the C-terminal helicase domain of full-length DHX33 (DHX33-1). Different from DHX33-1, which mainly localizes to the nucleus, DHX33-2 preferentially localizes to the cytoplasm. Through protein immunoprecipitation and RNA- immunoprecipitation analysis, we found that DHX33-2 interacts with DDX3, eIF3, hnRNPs, poly (A) binding protein, and a subset of mRNAs. Further RNA sequencing analysis showed that DHX33 binds to a subset of mRNAs important in cell proliferation. DHX33-2 stimulates the translation for specific mRNAs. Our study for the first time demonstrates the function of a short DHX33 variant in protein translation.
关键词	DHX33 isoform protein translation RNA helicase
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Guangdong Provincial Science and Technology Department[2017A020211024]
WOS研究方向	Cell Biology ; Physiology
WOS类目	Cell Biology ; Physiology
WOS记录号	WOS:000470174200080
出版者	WILEY
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/25222
专题	生命科学学院_生物系
作者单位	Southern Univ Sci & Technol, Dept Biol, 1088 Xueyuan Blvd, Shenzhen 518055, Peoples R China
第一作者单位	生物系
通讯作者单位	生物系
第一作者的第一单位	生物系
推荐引用方式 GB/T 7714	Su, Dan,Yuan, Baolei,Su, Chenjing,et al. A 54-kDa short variant of DHX33 functions in regulating mRNA translation[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(9):15308-15319.
APA	Su, Dan,Yuan, Baolei,Su, Chenjing,&Zhang, Yandong.(2019).A 54-kDa short variant of DHX33 functions in regulating mRNA translation.JOURNAL OF CELLULAR PHYSIOLOGY,234(9),15308-15319.
MLA	Su, Dan,et al."A 54-kDa short variant of DHX33 functions in regulating mRNA translation".JOURNAL OF CELLULAR PHYSIOLOGY 234.9(2019):15308-15319.