| 题名 | GSP-2, a polysaccharide extracted from Ganoderma sinense, is a novel toll-like receptor 4 agonist |
| 作者 | |
| 通讯作者 | Ko, Chun-Hay; Han, Xiao-Qiang |
| 发表日期 | 2019-08-23
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| DOI | |
| 发表期刊 | |
| ISSN | 1932-6203
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| 卷号 | 14期号:8 |
| 摘要 | Ganoderma sinense is a Chinese unique medicinal fungus that has been used in folk medicine for thousands of years. Polysaccharides are considered to be biologically active ingredients due to their immune-modulating functions. Previously we found that GSP-2, a new polysaccharide isolated from Ganoderma sinense, exerts an immunomodulatory effect in human peripheral blood mononuclear cells but the underlying mechanism is unclear. The present study aimed to investigate how GSP-2 triggers immunologic responses and the implicated signaling pathways. GSP-2 effects were investigated both in a macrophagic cell line, RAW264.7, and in primary macrophages. Moreover, the molecular basis of GSP-2 recognition by immune cells, and the consequent activation of signaling cascades, were explored by employing recombinant human HEK293-TLR-Blue clones, individually over-expressing various Toll-like receptors. GSP-2 dose-dependently induced the overexpression of Toll-like receptor 4 (TLR4) but did not affect the expression of other TLRs. Moreover, GSP-2 induced TNF alpha secretion in primary macrophages from wild-type, but not TLR4-knockout mice. In addition, GSP-2 upregulated TLR4 protein expression and activated the MAPK pathway in RAW246.7 macrophages. Finally, GSP-2 induced the production of the cytokines TNF alpha, IL1 beta, and IL6. Our data demonstrated that GSP-2 was specifically recognized by TLR4, promoting cytokine secretion and immune modulation in macrophages. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 第一
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| 资助项目 | Science and Technology Foundation of Shenzhen[JCYJ20180301170047864]
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| WOS研究方向 | Science & Technology - Other Topics
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| WOS类目 | Multidisciplinary Sciences
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| WOS记录号 | WOS:000485041400060
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| 出版者 | |
| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：13
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/25293 |
| 专题 | 南方科技大学第一附属医院 |
| 作者单位 | 1.Jinan Univ, Southern Univ Sci & Technol, Clin Med Coll 2, Shenzhen Peoples Hosp,Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China 2.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 3.Soochow Univ, Inst Biol & Med Sci, Suzhou, Jiangsu, Peoples R China 4.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Hubei, Peoples R China 5.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 6.Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen, Guangdong, Peoples R China |
| 第一作者单位 | 南方科技大学第一附属医院 |
| 第一作者的第一单位 | 南方科技大学第一附属医院 |
| 推荐引用方式 GB/T 7714 |
Liu, Kai-Sheng,Zhang, Cheng,Dong, Hong-Liang,et al. GSP-2, a polysaccharide extracted from Ganoderma sinense, is a novel toll-like receptor 4 agonist[J]. PLoS One,2019,14(8).
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| APA |
Liu, Kai-Sheng.,Zhang, Cheng.,Dong, Hong-Liang.,Li, Kai-Kai.,Han, Quan-Bin.,...&Han, Xiao-Qiang.(2019).GSP-2, a polysaccharide extracted from Ganoderma sinense, is a novel toll-like receptor 4 agonist.PLoS One,14(8).
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| MLA |
Liu, Kai-Sheng,et al."GSP-2, a polysaccharide extracted from Ganoderma sinense, is a novel toll-like receptor 4 agonist".PLoS One 14.8(2019).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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