Hierarchically structured microchip for point-of-care immunoassays with dynamic detection ranges

Mou, Lei1,3; Dong, Ruihua1; Hu, Binfeng1,3; Li, Zulan4; Zhang, Jiangjiang1,3; Jian, Xingyu1,2,3

2019-08-21

10.1039/c9lc00517j

LAB ON A CHIP   影响因子和分区

1473-0197

1473-0189

Point-of-care (POC) medical assays provide critical information to guide clinical therapy for a broad range of medical scenarios, such as resource-poor settings and specialty departments in hospitals. Even though many types of POC assays can be done in automated devices, these POC assays typically cannot well accommodate the multiplexed detection of biomarkers where a large dynamic range is needed. Here, we report a POC assay, which is both automated and suitable for detecting multiple biomarkers with dynamic detection ranges. We call it a dynamic multiplexed immunoassay (DMI). We control the concentrations of capture antibodies and the intensity of the readout signal to dynamically modulate the detection range of immunoassays (pg mL(-1) to mu g mL(-1)), leading to the multiplexed detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin 6 (IL-6) simultaneously in undiluted human serum samples. The POC assay allows the rapid and accurate detection of infection in patients.

SCI ; EI

英语

通讯

Chinese Academy of Sciences[QYZDJ-SSW-SLH039] ; Chinese Academy of Sciences[121D11KYSB20170026] ; Chinese Academy of Sciences[XDA16020902]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology

WOS:000478908200008

ROYAL SOC CHEMISTRY

20201708538522

Microprocessor chips ; Chemical detection

Chemistry:801

CHEMISTRY

Web of Science

1.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
4.306th Hosp Peoples Liberat Army, Reprod Ctr, 9 Anxiang Beili, Beijing 100101, Peoples R China

Mou, Lei,Dong, Ruihua,Hu, Binfeng,et al. Hierarchically structured microchip for point-of-care immunoassays with dynamic detection ranges[J]. LAB ON A CHIP,2019,19(16):2750-2757.

Mou, Lei,Dong, Ruihua,Hu, Binfeng,Li, Zulan,Zhang, Jiangjiang,&Jian, Xingyu.(2019).Hierarchically structured microchip for point-of-care immunoassays with dynamic detection ranges.LAB ON A CHIP,19(16),2750-2757.

Mou, Lei,et al."Hierarchically structured microchip for point-of-care immunoassays with dynamic detection ranges".LAB ON A CHIP 19.16(2019):2750-2757.