Rapid Equilibrated Colorimetric Detection of Protamine and Heparin: Recognition at the Nanoscale Liquid-Liquid Interface

Chen, Qinghan1,2; Li, Xiaoang2; Wang, Renjie2; Zeng, Fanxin3; Zhai, Jingying1; Xie, Xiaojiang2

2019-08-20

10.1021/acs.analchem.9b01654

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Demand for rapid quantitation of polyions such as heparin and protamine are ever growing. Previous paper-based and polymeric optical and electrochemical sensing devices required more than several hours for signal stabilization. Therefore, signals were acquired with fixed sample exposure time modes, which was time-consuming and technically demanding. We present here for the first time the optical detection of protamine and heparin in equilibrium mode with emulsified nanospheres. The method significantly shortens the response time from hours to typically less than 10 s and offers tunable, sensitive, and colorimetric detection within the clinically relevant range (10 to 100 mg/L) for heparin. The improved characteristics are attributable to the small size of the nanospheres (ca. 50 nm in diameter) as well as the reversible recognition at the nanoscale liquid-liquid interface. Detection of the anticoagulant heparin was also successfully demonstrated in human blood serum background.

SCI ; EI

英语

NI期刊

第一 ; 通讯

Shenzhen Municipal Science and Technology Innovation Council[JCYJ20170817105950724]

Chemistry

Chemistry, Analytical

WOS:000482545300010

AMER CHEMICAL SOC

20193407343784

Emulsification ; Liquids ; Polysaccharides ; Colorimetry

Nanotechnology:761 ; Chemical Operations:802.3 ; Organic Compounds:804.1 ; Organic Polymers:815.1.1 ; Solid State Physics:933 ; Optical Variables Measurements:941.4

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
3.Dazhou Cent Hosp, Dept Clin Res Ctr, Dazhou 635000, Peoples R China

Chen, Qinghan,Li, Xiaoang,Wang, Renjie,et al. Rapid Equilibrated Colorimetric Detection of Protamine and Heparin: Recognition at the Nanoscale Liquid-Liquid Interface[J]. ANALYTICAL CHEMISTRY,2019,91(16):10390-10394.

Chen, Qinghan,Li, Xiaoang,Wang, Renjie,Zeng, Fanxin,Zhai, Jingying,&Xie, Xiaojiang.(2019).Rapid Equilibrated Colorimetric Detection of Protamine and Heparin: Recognition at the Nanoscale Liquid-Liquid Interface.ANALYTICAL CHEMISTRY,91(16),10390-10394.

Chen, Qinghan,et al."Rapid Equilibrated Colorimetric Detection of Protamine and Heparin: Recognition at the Nanoscale Liquid-Liquid Interface".ANALYTICAL CHEMISTRY 91.16(2019):10390-10394.