| 题名 | Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions |
| 作者 | |
| 通讯作者 | Kejian Wang; Jixian Zhai |
| 共同第一作者 | Daoheng Hu; Yiming Yu; Chun Wang |
| 发表日期 | 2021-06-12
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| DOI | |
| 发表期刊 | |
| 卷号 | 33期号:9页码:2950–2964 |
| 摘要 | DNA methylation in the non-CG context is widespread in the plant kingdom and abundant in mammalian tissues such as the brain and pluripotent cells. Non-CG methylation in Arabidopsis thaliana is coordinately regulated by DOMAINS REARRANGED METHYLTRANSFERASE (DRM) and CHROMOMETHYLASE (CMT) proteins but has yet to be systematically studied in major crops due to diffificulties in obtaining genetic materials. Here, utilizing the highly effificient multiplex
CRISPR-Cas9 genome-editing system, we created single- and multiple-knockout mutants for all the nine DNA methyltrans ferases in rice (Oryza sativa) and profifiled their whole-genome methylation status at single-nucleotide resolution.
Surprisingly, the simultaneous loss of DRM2, CHROMOMETHYLASE3 (CMT2), and CMT3 functions, which completely erases all non-CG methylation in Arabidopsis, only partially reduced it in rice. The regions that remained heavily methyl ated in non-CG contexts in the rice Os-dcc (Osdrm2/cmt2/cmt3a) triple mutant had high GC contents. Furthermore, the
residual non-CG methylation in the Os-dcc mutant was eliminated in the Os-ddccc (Osdrm2/drm3/cmt2/cmt3a/cmt3b) quintuple mutant but retained in the Os-ddcc (Osdrm2/drm3/cmt2/cmt3a) quadruple mutant, demonstrating that OsCMT3b maintains non-CG methylation in the absence of other major methyltransferases. Our results showed that OsCMT3b is subfunctionalized to accommodate a distinct cluster of non-CG-methylated sites at highly GC-rich regions in the rice genome. |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 通讯
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| WOS记录号 | WOS:000702165300010
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| 来源库 | 人工提交
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| 引用统计 |
被引频次[WOS]：19
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/253219 |
| 专题 | 南方科技大学-北京大学植物与食品联合研究所 生命科学学院 生命科学学院_生物系 |
| 作者单位 | 1.School of Life Sciences & Institute of Plant and Food Science & Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China 2.College of Horticulture, South China Agricultural University, Guangzhou 510642, China 3.State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China 4.gzhou, China 4 Laboratory of Genetics & Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, USA 5.Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China |
| 第一作者单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 通讯作者单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 第一作者的第一单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 推荐引用方式 GB/T 7714 |
Daoheng Hu,Yiming Yu,Chun Wang,et al. Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions[J]. THE PLANT CELL,2021,33(9):2950–2964.
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| APA |
Daoheng Hu.,Yiming Yu.,Chun Wang.,Yanping Long.,Yue Liu.,...&Jixian Zhai.(2021).Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions.THE PLANT CELL,33(9),2950–2964.
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| MLA |
Daoheng Hu,et al."Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions".THE PLANT CELL 33.9(2021):2950–2964.
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| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | 操作 | |
| 2021.Plant.Cell-2.pd（2550KB） | -- | -- | 开放获取 | -- | 浏览 | |
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