Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota

Zhong, Ruting1; Chen, Lanbin1; Liu, Yuanyuan1; Xie, Shouxia2,3; Li, Sumei2,3; Liu, Bin1,4; Zhao, Chao1,4

2022

10.1016/j.fshw.2021.07.019

FOOD SCIENCE AND HUMAN WELLNESS   影响因子和分区

2213-4530

This research aimed to investigate the antidiabetic activity, underlying mechanisms, and gut microbiota regulation of aloin. The insulin-resistant HepG2 (IR-HepG2) cell model and the type 2 diabetic (T2D) mouse model were successfully established using dexamethasone and a high-fat high-sucrose diet with low-dose streptozotocin, respectively. Aloin intervention increased glucose consumption and stimulated the activity of hexokinase and pyruvate dehydrogenase in IR-HepG2 cells. Additionally, it diminished the weight loss, reduced fasting blood glucose levels and hemoglobin A1c activity, and promoted glucose tolerance and fasting serum insulin activity in T2D mice. Histopathological analysis of the liver indicated hepatic protection by aloin. Additionally, aloin treatment inhibited the protein expression of c-Jun N-terminal kinases and activated that of IRS1/PI3K/Akt in the liver. Moreover, aloin modulated the bacterial community in the gut by raising the abundance of Bacteroidota and reducing the richness of Firmicutes, Proteobacteria, and Actinobacteriota. Thus, aloin ameliorated IR via activating IRS1/PI3K/Akt signaling pathway and regulating the gut microbiota, and it may be promising candidate as functional food for diabetic therapy. (C) 2021 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Aloin Antidiabetic Gut microbiota Insulin signaling pathway

SCI

英语

通讯

Key Project of the Natural Science Foundation of Fujian Province[2020J02032] ; Double First-Class Construction Plan of Fujian Agriculture and Forestry University[KSYLX013] ; Shenzhen Key Medical Discipline Construction Fund[SZXK059]

Food Science & Technology ; Nutrition & Dietetics

Food Science & Technology ; Nutrition & Dietetics

WOS:000695531000019

KEAI PUBLISHING LTD

Web of Science

1.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Peoples R China
2.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Pharmacol, Shenzhen 518020, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
4.Minist Educ, Engn Res Ctr Fujian Taiwan Special Marine Food Pr, Fuzhou 350002, Peoples R China

Zhong, Ruting,Chen, Lanbin,Liu, Yuanyuan,et al. Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota[J]. FOOD SCIENCE AND HUMAN WELLNESS,2022,11(1).

Zhong, Ruting.,Chen, Lanbin.,Liu, Yuanyuan.,Xie, Shouxia.,Li, Sumei.,...&Zhao, Chao.(2022).Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota.FOOD SCIENCE AND HUMAN WELLNESS,11(1).

Zhong, Ruting,et al."Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota".FOOD SCIENCE AND HUMAN WELLNESS 11.1(2022).