Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure

Huang, Jiahuang; Tang, Donge; Zheng, Fengping; Xu, Huixuan; Dai, Yong

2021-09-13

10.1186/s12882-021-02445-4

BMC NEPHROLOGY   影响因子和分区

1471-2369

Background Post-translational modifications (PTMs) are at the heart of many cellular signaling events, which changes the function of protein. Crotonylation, one of the most important and common PTMs, plays a crucial role in the regulation of various biological processes. However, no study has evaluated the role of lysine crotonylation modification in chronic renal failure (CRF) patients. Methods Here, we comparatively evaluated the crotonylation proteome of normal controls and chronic renal failure patients using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with highly sensitive immune-affinity purification. Results A total of 1109 lysine modification sites were identified, of which 772 sites were up-regulated and 69 sites were down-regulated. This suggested that crotonylation modification maintains high levels in the patients with chronic renal failure. Gene ontology(GO) enrichment analysis showed that the crotonylated proteins were significantly enriched in the platelet alpha granule lumen, platelet degradulation, and cell adhesion molecule binding. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG)-based functional enrichment analysis in the Kyoto encyclopedia showed that crotonylated protein was enriched in CD36, which is closely linked to renal failure. Conclusions This is the first report of the global crotonylation proteome in chronic renal failure patients. Crotonylation of histone and non-histone may play important roles in delaying the continuous deterioration of renal function in patients with chronic renal failure.

Crotonylation Modification Epigenetics Chronic renal failure Mass Spectrometry Failure

SCI

英语

第一 ; 通讯

Science and Technology Planning Project of Guangdong Pronvince, China[2017B020209001] ; National Natural Science Foudation of China[81671596] ; Science and Technology plan of Shenzhen[JCY20190807153405508]

Urology & Nephrology

Urology & Nephrology

WOS:000698201600002

BMC

Web of Science

Southern Univ Sci & Technol, Shenzhen Engn Res Ctr Autoimmune Dis, Shenzhen Peoples Hosp,Affiliated Hosp 1, Clin Med Res Ctr,Guangdong Prov Engn Res Ctr Auto, Shenzhen 518020, Guangdong, Peoples R China

Huang, Jiahuang,Tang, Donge,Zheng, Fengping,et al. Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure[J]. BMC NEPHROLOGY,2021,22(1).

Huang, Jiahuang,Tang, Donge,Zheng, Fengping,Xu, Huixuan,&Dai, Yong.(2021).Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure.BMC NEPHROLOGY,22(1).

Huang, Jiahuang,et al."Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure".BMC NEPHROLOGY 22.1(2021).