Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals

Luo, Lingjie1,2,3; Liang, Wenhua1,2,3; Pang, Jianfeng1,2,3; Xu, Gang4,5; Chen, Yingying1; Guo, Xinrong1,2; Wang, Xin1,2; Zhao, Yi1; Lai, Yangdian1; Liu, Yang4,5; Li, Bin1; Su, Bing1,3; Zhang, Shuye6; Baniyash, Michal7; Shen, Lei1; Chen, Lei1,3; Ling, Yun6; Wang, Ying1; Liang, Qiming1,2; Lu, Hongzhou6; Zhang, Zheng4,5; Wang, Feng1,2,3

2021-09-28

10.1038/s41421-021-00321-x

CELL DISCOVERY   影响因子和分区

2056-5968

SARS-CoV-2 outbreak has been declared by World Health Organization as a worldwide pandemic. However, there are many unknowns about the antigen-specific T-cell-mediated immune responses to SARS-CoV-2 infection. Here, we present both single-cell TCR-seq and RNA-seq to analyze the dynamics of TCR repertoire and immune metabolic functions of blood T cells collected from recently discharged COVID-19 patients. We found that while the diversity of TCR repertoire was increased in discharged patients, it returned to basal level similar to 1 week after becoming virus-free. The dynamics of T cell repertoire correlated with a profound shift of gene signatures from antiviral response to metabolism adaptation. We also demonstrated that the top expanded T cell clones (similar to 10% of total T cells) display the key anti-viral features in CD8(+) T cells, confirming a critical role of antigen-specific T cells in fighting against SARS-CoV-2. Our work provides a basis for further analysis of adaptive immunity in COVID-19 patients, and also has implications in developing a T-cell-based vaccine for SARS-CoV-2.

SCI

英语

通讯

National Key Research and Development Program of China[SQ2018YFA090045-01] ; National Natural Science Foundation of China[81771739,82071852] ; Technology Committee of Shanghai Municipality["18JC1414100",20410713800,"20JC1410200"] ; Shanghai Academic Research Leader Project[2018XD1403300] ; National Science Fund for Distinguished Young Scholars[82025022] ; Central Charity Fund of Chinese Academy of Medical Science[2020-PT310-009] ; Cultivation Project of Shanghai Major Infectious Disease Research Base[20dz2210500]

Cell Biology

Cell Biology

WOS:000700418100001

SPRINGERNATURE

Web of Science

1.Shanghai Jiao Tong Univ, Shanghai Inst Immunol, Dept Immunol & Microbiol, State Key Lab Oncogenes & Related Genes,Sch Med, Shanghai, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Childrens Hosp, Res Ctr Translat Med, Sch Med, Shanghai, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Ctr Microbiota & Immunol Dis, Shanghai Inst Immunol,Sch Med, Shanghai, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Inst Hepatol, Shenzhen, Guangdong, Peoples R China
5.Southern Univ Sci & Technol, Affiliated Hosp 2, Sch Med, Shenzhen, Guangdong, Peoples R China
6.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
7.Hebrew Univ Jerusalem, Fac Med, Lautenberg Ctr Immunol & Canc Res, Israel Canada Med Res Inst, Jerusalem, Israel

Luo, Lingjie,Liang, Wenhua,Pang, Jianfeng,et al. Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals[J]. CELL DISCOVERY,2021,7(1).

Luo, Lingjie.,Liang, Wenhua.,Pang, Jianfeng.,Xu, Gang.,Chen, Yingying.,...&Wang, Feng.(2021).Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals.CELL DISCOVERY,7(1).

Luo, Lingjie,et al."Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals".CELL DISCOVERY 7.1(2021).