Detection of a clinical carbapenem-resistant Citrobacter portucalensis strain and the dissemination of C. portucalensis in clinical settings

Cao, Xiaoli1; Xie, Hui1; Huang, Doudou2; Zhou, Wanqing1; Liu, Yang3,4; Shen, Han1; Zhou, Kai3,4

2021-12-01

10.1016/j.jgar.2021.04.027

Journal of Global Antimicrobial Resistance   影响因子和分区

2213-7165

2213-7173

["Objectives: A clinical carbapenem-resistant Citrobacter portucalensis was characterised by whole-genome sequencing (WGS).","Methods: Strain 3839 was identified by VITEK (R) 2.0 and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antimicrobial susceptibility testing was performed by microbroth dilution. WGS followed by bioinformatics analysis was performed.","Results: Strain 3839 was initially identified as Citrobacter freundii by VITEK and MALDI-TOF/MS, and was subsequently demonstrated to be C. portucalensis by WGS analysis. Through average nucleotide identity analysis, we detected 55 C. portucalensis genomes misidentified as C. freundii in GenBank, and at least 22 were clinically-associated, suggesting that the occurrence of C. portucalensis in the clinical setting might be underestimated by the conventional method. Strain 3839 was extensively drug-resistant and the presence of multiple resistance determinants was detected by WGS, including bla(NDM-1), bla(SHV-12), bla(CMY-150), bla(OXA-1), qnrB9, qnrA1, aac(6')-Ib-cr, aph(6)-Id_1, aph(3 '')-Ib, tetA, tet34 and catB3. A total of 45 insertion sequence (IS) elements, 8 phages and 1 integron gene cassette were identified. The bla(NDM-1) gene was carried by an IncX3 plasmid that was identical to a plasmid detected in a C. freundii strain. The genetic context of bla(NDM-1) was IS30-bla(NDM-1)-ble(MBL)-trpF-dsbD-cutA1-groES-groEL-IS91.","Conclusion: To our knowledge, this is the first report of carbapenem-resistant C. portucalensis isolated from a clinical sample. The bla(NDM-1) gene carried by C. portucalensis may transmit among Citrobacter spp. mediated by plasmids. Together with underestimation of its clinical occurrence caused by misidentification, our study warrants the necessity of preventing the dissemination of such emerging drug-resistant species in clinical settings. (C) 2021 Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy."]

linical strains Citrobacter portucalensis Carbapenem resistance NDM-1 Clinical strains

SCI

英语

通讯

National Natural Science Foundation of China[81902124]

Infectious Diseases ; Pharmacology & Pharmacy

Infectious Diseases ; Pharmacology & Pharmacy

WOS:000701661400014

ELSEVIER SCI LTD

Web of Science

1.Nanjing Univ, Med Sch, Affiliated Hosp, Dept Lab Med,Nanjing Drum Tower Hosp, Nanjing, Jiangsu, Peoples R China
2.Nanjing Univ, Med Sch, Affiliated Drum Tower Hosp, Dept Med Records & Stat Room, Nanjing 210008, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen Inst Resp Dis, Shenzhen, Peoples R China
4.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen, Peoples R China

Cao, Xiaoli,Xie, Hui,Huang, Doudou,et al. Detection of a clinical carbapenem-resistant Citrobacter portucalensis strain and the dissemination of C. portucalensis in clinical settings[J]. Journal of Global Antimicrobial Resistance,2021,27.

Cao, Xiaoli.,Xie, Hui.,Huang, Doudou.,Zhou, Wanqing.,Liu, Yang.,...&Zhou, Kai.(2021).Detection of a clinical carbapenem-resistant Citrobacter portucalensis strain and the dissemination of C. portucalensis in clinical settings.Journal of Global Antimicrobial Resistance,27.

Cao, Xiaoli,et al."Detection of a clinical carbapenem-resistant Citrobacter portucalensis strain and the dissemination of C. portucalensis in clinical settings".Journal of Global Antimicrobial Resistance 27(2021).