Clinical Evaluation of a Metagenomics-Based Assay for Pneumonia Management

Zhan, Yangqing1; Xu, Teng2,3,4; He, Fusheng2; Guan, Wei-jie1,5; Li, Zhengtu1; Li, Shaoqiang1; Xie, Mingzhou2; Li, Xiaolei2; Chen, Rongchang1,6; Cheng, Linling1; Zhong, Nanshan1; Ye, Feng1

2021-09-16

10.3389/fmicb.2021.751073

FRONTIERS IN MICROBIOLOGY   影响因子和分区

1664-302X

Clinical value of metagenomic next-generation sequencing (mNGS) in pneumonia management is still controversial. A prospective study was conducted to evaluate the clinical impact of PneumoSeq in 57 immunocompetent (ICO) and 75 immunocompromised (ICH) pneumonia patients. The value of PneumoSeq for both etiological and clinical impact investigation in pneumonia was assessed. Among the 276 potential pathogens detected with PneumoSeq in our cohort, 251 (90.9%) were cross-validated. Clinical diagnoses of the causative pathogens were obtained for 97 patients, 90.7% of which were supported by PneumoSeq. Compared to conventional testing, PneumoSeq suggested potentially missed diagnoses in 16.7% of cases (22/132), involving 48 additional pathogenic microorganisms. In 58 (43.9%) cases, PneumoSeq data led to antimicrobial treatment de-escalation (n = 12 in ICO, n = 18 in ICH) and targeted treatment initiation (n = 7 in ICO, n = 21 in ICH). The PneumoSeq assay benefited the diagnosis and clinical management of both ICH and ICO pneumonia patients in real-world settings.

pneumonia metagenomic next-generation sequence pathogens cytomegalovirus pneumocystis jirovecii

SCI

英语

其他

National Major Science and Technology Projects[2017ZX09304005002] ; Guangdong Province Major Area Research and Development Projects[2018B020241002]

Microbiology

Microbiology

WOS:000702120300001

FRONTIERS MEDIA SA

Web of Science

1.Guangzhou Med Univ, Natl Clin Res Ctr Resp Dis, Guangzhou Inst Resp Hlth,Affiliated Hosp 1, State Key Lab Resp Dis,Dept Pulm & Crit Care Med, Guangzhou, Peoples R China
2.Vis Med Co Ltd, Guangzhou, Peoples R China
3.Yunnan Agr Univ, Key Lab Anim Gene Editing & Anim Cloning Yunnan P, Kunming, Yunnan, Peoples R China
4.Yunnan Agr Univ, Coll Vet Med, Kunming, Yunnan, Peoples R China
5.Guangzhou Med Univ, Dept Thorac Surg, Guangzhou Inst Resp Hlth, Affiliated Hosp 1, Guangzhou, Peoples R China
6.Jinan Univ, Dept Resp & Crit Care Med, Shenzhen Inst Resp Dis,Affiliated Hosp 1, Southern Univ Sci & Technol,Clin Med Coll 2,Shenz, Shenzhen, Peoples R China

Zhan, Yangqing,Xu, Teng,He, Fusheng,et al. Clinical Evaluation of a Metagenomics-Based Assay for Pneumonia Management[J]. FRONTIERS IN MICROBIOLOGY,2021,12.

Zhan, Yangqing.,Xu, Teng.,He, Fusheng.,Guan, Wei-jie.,Li, Zhengtu.,...&Ye, Feng.(2021).Clinical Evaluation of a Metagenomics-Based Assay for Pneumonia Management.FRONTIERS IN MICROBIOLOGY,12.

Zhan, Yangqing,et al."Clinical Evaluation of a Metagenomics-Based Assay for Pneumonia Management".FRONTIERS IN MICROBIOLOGY 12(2021).