Elastic modulus and migration capability of drug treated leukemia cells K562

Wang, Kui1,2; Xue, Yuntian1; Peng, Ying1; Pang, Xiangchao4; Zhang, Yuanjun1; Ruiz-Ortega, L. I.1; Tian, Ye1; Ngan, A. H. W.2; Tang, Bin1,3

2019-08-13

10.1016/j.bbrc.2019.06.024

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Leukemia is a commonly seen disease caused by abnormal differentiation of hematopoietic stem cells and blasting in bone marrow. Despite drugs are used to treat the disease clinically, the influence of these drugs on leukemia cells' biomechanical properties, which are closely related to complications like leukostasis or infiltration, is still unclear. Due to non-adherent and viscoelastic nature of leukemia cells, accurate measurement of their elastic modulus is still a challenging issue. In this study, we adopted rate jump method together with optical tweezers indentation to accurately measure elastic modulus of leukemia cells K562 after phorbol 12-myristate 13-acetate (PMA), all-trans retinoic acid (ATRA), Cytoxan (CTX), and Dexamethasone (DEX) treatment, respectively. We found that compared to control sample, K562 cells treated by PMA showed nearly a threefold increase in elastic modulus. Transwell experiment results suggested that the K562 cells treated with PMA have the lowest migration capability. Besides, it was shown that the cytoskeleton protein gene alpha-tubulin and vimentin have a significant increase in expression after PMA treatment by OCR. The results indicate that PMA has a significant influence on protein expression, stiffness, and migration ability of the leukemia cell K562, and may also play an important role in the leukostasis in leukemia. (C) 2019 Elsevier Inc. All rights reserved.

Leukemia cells Stiffness PMA Vimentin Optical tweezers

SCI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Committee[JCYJ20160517160827379] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170817111312887] ; Shenzhen Science and Technology Innovation Committee[ZDSYS20140509142721429]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000476580800027

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
2.Univ Hong Kong, Dept Mech Engn, Hong Kong, Peoples R China
3.Shenzhen Key Lab Cell Microenvironm, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen, Peoples R China
4.Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China

Wang, Kui,Xue, Yuntian,Peng, Ying,et al. Elastic modulus and migration capability of drug treated leukemia cells K562[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,516(1):177-182.

Wang, Kui.,Xue, Yuntian.,Peng, Ying.,Pang, Xiangchao.,Zhang, Yuanjun.,...&Tang, Bin.(2019).Elastic modulus and migration capability of drug treated leukemia cells K562.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,516(1),177-182.

Wang, Kui,et al."Elastic modulus and migration capability of drug treated leukemia cells K562".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 516.1(2019):177-182.