南方科技大学知识苑(SUSTech KC): Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics

题名	Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics
作者	Li，Xuanyu; Feng，Qiang; Han，Ziwei; Jiang，Xingyu
通讯作者	Jiang，Xingyu
发表日期	2021
DOI	10.1016/j.cjche.2021.02.009
发表期刊	CHINESE JOURNAL OF CHEMICAL ENGINEERING 影响因子和分区
ISSN	1004-9541
EISSN	2210-321X
卷号	38页码:216-220
摘要	Most non-viral carriers for in vitro delivery of nucleic acids suffer from low efficiency of introducing mRNA and other nucleic acids, especially large mRNA. Cas9 protein is the nuclease part of the powerful gene-editing tool, CRISPR/Cas9 system, Cas9 mRNA is particularly large, thus presents a big challenge for delivery. We assembled a multilayered biodegradable nanocarrier to load Cas9 mRNA inside to protect Cas9 mRNA from degradation. We used a microfluidic chip to synthesize a small, positively charged, and degradable core to attract negatively charged Cas9 mRNA. The microfluidic assembly allows the core to be small enough to incorporate into a cationic liposome. The multilayered nanocarriers elevated the delivery efficiency of Cas9 mRNA by over 2 folds and increased the expression by over 5 folds compared to commercially used non-viral carriers. In addition, the multilayered nanocarriers do not require reduced serum medium for transfection. When using the standard complete medium for transfection, the multilayered nanocarriers could increase the expression of Cas9 mRNA by over 15 folds compared to commercially used non-viral carriers. The co-delivery of Cas9 mRNA and sgRNA via LRC elevated the gene-editing efficiency by 3 folds compared to that via commercially used non-viral carriers. Based on the higher transfection efficiency of Cas9 mRNA/sgRNA than commercially used non-viral carriers, these multilayered nanocarriers may have a good prospect as efficient commercial delivery carriers for Cas9 mRNA/sgRNA and other nucleic acids.
关键词	Biomedical engineering CRISPR/Cas9 system Microfluidics Nanotechnology Poly(lactic-co-glycolic acid)
相关链接	[Scopus记录]
收录类别	EI ; SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China[21761142006,21535001,81730051] ; Shenzhen Science and Technology Program[KQTD20190929172743294] ; National Key R&D Program of China[2018YFA0902600] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039] ; Shenzhen Bay Laboratory[SZBL2019062801004]
WOS研究方向	Engineering
WOS类目	Engineering, Chemical
WOS记录号	WOS:000724098200021
出版者	CHEMICAL INDUSTRY PRESS CO LTD
EI入藏号	20213910962813
EI主题词	Biomedical engineering ; Biomolecules ; Efficiency ; Genes ; Nucleic acids
EI分类号	Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Microfluidics:632.5.1 ; Biochemistry:801.2 ; Organic Compounds:804.1 ; Production Engineering:913.1
ESI学科分类	CHEMISTRY
Scopus记录号	2-s2.0-85115952328
来源库	Scopus
引用统计	被引频次[WOS]：6
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/253564
专题	工学院_生物医学工程系
作者单位	Shenzhen Bay Laboratory,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Li，Xuanyu,Feng，Qiang,Han，Ziwei,et al. Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,2021,38:216-220.
APA	Li，Xuanyu,Feng，Qiang,Han，Ziwei,&Jiang，Xingyu.(2021).Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics.CHINESE JOURNAL OF CHEMICAL ENGINEERING,38,216-220.
MLA	Li，Xuanyu,et al."Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics".CHINESE JOURNAL OF CHEMICAL ENGINEERING 38(2021):216-220.