| 题名 | Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2 |
| 作者 | Malik, Vikas1,2,3,4; Glaser, Laura, V5; Zimmer, Dennis1,2,3,4; Velychko, Sergiy6; Weng, Mingxi7; Holzner, Markus7; Arend, Marius7; Chen, Yanpu1,3,4; Srivastava, Yogesh1,2,3,4; Veerapandian, Veeramohan1,2,3,4,8; Shah, Zahir2,3,4; Esteban, Miguel A.1,3,4,9,10,11; Wang, Huating12; Chen, Jiekai1,3,4,11; Schoeler, Hans R.6,13; Hutchins, Andrew P.14  ; Meijsing, Sebastiaan H.5; Pott, Sebastian15; Jauch, Ralf1,3,4,7
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| 通讯作者 | Jauch, Ralf |
| 发表日期 | 2019-08-02
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| DOI | |
| 发表期刊 | |
| ISSN | 2041-1723
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| 卷号 | 10 |
| 摘要 | Oct4, along with Sox2 and Klf4 (SK), can induce pluripotency but structurally similar factors like Oct6 cannot. To decode why Oct4 has this unique ability, we compare Oct4-binding, accessibility patterns and transcriptional waves with Oct6 and an Oct4 mutant defective in the dimerization with Sox2 (Oct4(defSox2)). We find that initial silencing of the somatic program proceeds indistinguishably with or without Oct4. Oct6 mitigates the mesenchymal-to-epithelial transition and derails reprogramming. These effects are a consequence of differences in genome-wide binding, as the early binding profile of Oct4(defSox2) resembles Oct4, whilst Oct6 does not bind pluripotency enhancers. Nevertheless, in the Oct6-SK condition many otherwise Oct4-bound locations become accessible but chromatin opening is compromised when Oct4(defSox2) occupies these sites. We find that Sox2 predominantly facilitates chromatin opening, whilst Oct4 serves an accessory role. Formation of Oct4/Sox2 heterodimers is essential for pluripotency establishment; however, reliance on Oct4/Sox2 heterodimers declines during pluripotency maintenance. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI论文
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| 学校署名 | 其他
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| 资助项目 | Deutsche Forschungsgemeinschaft[ME4154/4-1]
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| WOS研究方向 | Science & Technology - Other Topics
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| WOS类目 | Multidisciplinary Sciences
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| WOS记录号 | WOS:000478576500009
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| 出版者 | |
| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：51
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/25376 |
| 专题 | 生命科学学院_生物系 |
| 作者单位 | 1.Guangzhou Med Univ, CAS Key Lab Regenerat Biol, Joint Sch Life Sci, Guangzhou Inst Biomed & Hlth,Chinese Acad Sci, Guangzhou 510530, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Guangdong Prov Key Lab Stem Cell Arid Regenerat M, Guangzhou 510530, Guangdong, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China 5.Max Planck Inst Mol Genet, Ihnestr 63-73, D-14195 Berlin, Germany 6.Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany 7.Univ Hong Kong, Sch Biomed Sci, Li Ka Shing Fac Med, Hong Kong, Peoples R China 8.Southern Med Univ, Dept Dev Biol, Sch Basic Med Sci, Guangzhou 510515, Guangdong, Peoples R China 9.Chinese Acad Sci, Lab RNA Chromatin & Human Dis, Key Lab Regenerat Biol, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China 10.Chinese Acad Sci, Guangdong Prov Key Lab Stem Cells & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China 11.GRMH GDL, Guangzhou 510005, Guangdong, Peoples R China 12.Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China 13.Univ Munster, Med Fac, D-48149 Munster, Germany 14.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China 15.Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 |
Malik, Vikas,Glaser, Laura, V,Zimmer, Dennis,et al. Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2[J]. Nature Communications,2019,10.
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| APA |
Malik, Vikas.,Glaser, Laura, V.,Zimmer, Dennis.,Velychko, Sergiy.,Weng, Mingxi.,...&Jauch, Ralf.(2019).Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.Nature Communications,10.
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| MLA |
Malik, Vikas,et al."Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2".Nature Communications 10(2019).
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| 条目包含的文件 | ||||||
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