Overexpression of Cinnamoyl-CoA Reductase 2 in Brassica napus Increases Resistance to Sclerotinia sclerotiorum by Affecting Lignin Biosynthesis

Liu, Dongxiao1; Wu, Jian1; Lin, Li1; Li, Panpan1; Li, Saifen1; Wang, Yue1; Li, Jian1; Sun, Qinfu2; Liang, Jiansheng3; Wang, Youping1,2

2021-09-23

10.3389/fpls.2021.732733

Frontiers in Plant Science   影响因子和分区

1664-462X

Sclerotinia sclerotiorum causes severe yield and economic losses for many crop and vegetable species, especially Brassica napus. To date, no immune B. napus germplasm has been identified, giving rise to a major challenge in the breeding of Sclerotinia resistance. In the present study, we found that, compared with a Sclerotinia-susceptible line (J902), a Sclerotinia-resistant line (J964) exhibited better xylem development and a higher lignin content in the stems, which may limit the invasion and spread of S. sclerotiorum during the early infection period. In addition, genes involved in lignin biosynthesis were induced under S. sclerotiorum infection in both lines, indicating that lignin was deposited proactively in infected tissues. We then overexpressed BnaC.CCR2.b, which encodes the first rate-limiting enzyme (cinnamoyl-CoA reductase) that catalyzes the reaction of lignin-specific pathways, and found that overexpression of BnaC.CCR2.b increased the lignin content in the stems of B. napus by 2.28-2.76% under normal growth conditions. We further evaluated the Sclerotinia resistance of BnaC.CCR2.b overexpression lines at the flower-termination stage and found that the disease lesions on the stems of plants in the T-2 and T-3 generations decreased by 12.2-33.7% and 32.5-37.3% compared to non-transgenic control plants, respectively, at 7days post-inoculation (dpi). The above results indicate that overexpression of BnaC.CCR2.b leads to an increase in lignin content in the stems, which subsequently leads to increased resistance to S. sclerotiorum. Our findings demonstrate that increasing the lignin content in the stems of B. napus is an important strategy for controlling Sclerotinia.

Brassica napus Sclerotinia sclerotiorum cinnamoyl-CoA reductase lignin resistance

SCI

英语

其他

National Natural Science Foundation of China[32072020,31901504,32172019] ; Jiangsu Agricultural Science and Technology Innovation Fund[CX(20)3120] ; National Science Foundation of Jiangsu Province["BE2018356","BK20190894"] ; Project of Special Funding for Crop Science Discipline Development[yzuxk202006]

Plant Sciences

Plant Sciences

WOS:000704207200001

FRONTIERS MEDIA SA

Web of Science

1.Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Yangzhou, Jiangsu, Peoples R China
2.Yangzhou Univ, Jiangsu Key Lab Crop Genom & Mol Breeding, Yangzhou, Jiangsu, Peoples R China
3.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China

Liu, Dongxiao,Wu, Jian,Lin, Li,et al. Overexpression of Cinnamoyl-CoA Reductase 2 in Brassica napus Increases Resistance to Sclerotinia sclerotiorum by Affecting Lignin Biosynthesis[J]. Frontiers in Plant Science,2021,12.

Liu, Dongxiao.,Wu, Jian.,Lin, Li.,Li, Panpan.,Li, Saifen.,...&Wang, Youping.(2021).Overexpression of Cinnamoyl-CoA Reductase 2 in Brassica napus Increases Resistance to Sclerotinia sclerotiorum by Affecting Lignin Biosynthesis.Frontiers in Plant Science,12.

Liu, Dongxiao,et al."Overexpression of Cinnamoyl-CoA Reductase 2 in Brassica napus Increases Resistance to Sclerotinia sclerotiorum by Affecting Lignin Biosynthesis".Frontiers in Plant Science 12(2021).