The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]

Zhang，Daowei1,2; Tan，Yongjun3; Dong，Fang2; Zhang，Ya2; Huang，Yanlan2; Zhou，Yizhou2; Zhao，Zhi Jian4; Yin，Qin2; Xie，Xuehua2; Gao，Xiewang2; Zhang，Chaofan2; Tu，Naimei1

2021-09-23

10.3389/fpls.2021.688707

Frontiers in Plant Science   影响因子和分区

1664-462X

1664-462X

IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement.

anthocyanin biosynthesis early response gene multiple members purple-fleshed sweetpotato R2R3-MYB transcriptome

SCI

英语

其他

National Key Research and Development Plan["2018YFD1000700","2018YFD1000705"] ; Innovation Fund Project of Hunan Agricultural Science and Technology[2020CX03-5] ; National Natural Science Foundation of Hunan Province[2018JJ3319] ; Provincial Key Research and Development Plan of Hunan Province[2020NK2042] ; China Agriculture Research System of MOF and MARA[CARS-10-C-16-2021]

Plant Sciences

Plant Sciences

WOS:000705905100001

FRONTIERS MEDIA SA

2-s2.0-85116682087

Scopus

1.College of Agronomy,Hunan Agricultural University,Changsha,China
2.Crop Research Institute,Hunan Academy of Agricultural Sciences,Changsha,China
3.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China
4.Dryland Crop Research Institute,Shao Yang Academy of Agriculture Science,Shaoyang,China

Zhang，Daowei,Tan，Yongjun,Dong，Fang,et al. The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam][J]. Frontiers in Plant Science,2021,12.

Zhang，Daowei.,Tan，Yongjun.,Dong，Fang.,Zhang，Ya.,Huang，Yanlan.,...&Tu，Naimei.(2021).The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam].Frontiers in Plant Science,12.

Zhang，Daowei,et al."The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]".Frontiers in Plant Science 12(2021).