Development and quantitation of pseudomonas aeruginosa biofilms after in vitro cultivation in flow-reactors

Zhang，Yingdan1; Zhao，Jingru2; Cheng，Hang1; Wang，Jing1; Yang，Liang1,3; Liang，Haihua2

2021-08-20

10.21769/BioProtoc.4194

Bio-protocol   影响因子和分区

2331-8325

Characterization of biofilm formation and metabolic activities is critical to investigating biofilm interactions with environmental factors and illustrating biofilm regulatory mechanisms. An appropriate in vitro model that mimics biofilm in vivo habitats therefore demands accurate quantitation and investigation of biofilm-associated activities. Current methodologies commonly involve static biofilm setups (such as biofilm assays in microplates, bead biofilms, or biofilms on glass-slides) and fluidic flow biofilm systems (such as drip-flow biofilm reactors, 3-channel biofilm reactors, or tubing biofilm reactors). Continuous flow systems take into consideration the contribution of hydrodynamic shear forces, nutrient supply, and physical transport of dispersed cells, which define the habitat for biofilm development in most natural and engineered systems. This protocol describes the assembly of 3 flow-system setups to cultivate Pseudomonas aeruginosa PAO1 and Shewanella oneidensis MR-1 model biofilms, including the respective quantitation and observation approaches. The standardized flow systems promise productive and reproducible biofilm experimental results, which can be further modified according to specific research projects.

Biofilm characterization Fluid-flow biofilm reactors Pseudomonas aeruginosa

ESCI

英语

第一 ; 通讯

WOS:000687827100017

2-s2.0-85116245484

Scopus

1.School of Medicine,Southern University of Science and Technology,Shenzhen,China
2.College of Life Sciences,Northwest University,Xi'an,ShaanXi,China
3.Shenzhen Key Laboratory of Gene Regulation and Systems Biology,Southern University of Science and Technology,Shenzhen,China

Zhang，Yingdan,Zhao，Jingru,Cheng，Hang,et al. Development and quantitation of pseudomonas aeruginosa biofilms after in vitro cultivation in flow-reactors[J]. Bio-protocol,2021,11(16).

Zhang，Yingdan,Zhao，Jingru,Cheng，Hang,Wang，Jing,Yang，Liang,&Liang，Haihua.(2021).Development and quantitation of pseudomonas aeruginosa biofilms after in vitro cultivation in flow-reactors.Bio-protocol,11(16).

Zhang，Yingdan,et al."Development and quantitation of pseudomonas aeruginosa biofilms after in vitro cultivation in flow-reactors".Bio-protocol 11.16(2021).