Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform

Xing, Fuqiang1,2,3,6,7; Liu, Yu-Cheng1; Huang, Shigao1; Lyu, Xueying1,2; Su, Sek Man1,2; Chan, Un In1,2; Wu, Pei-Chun1; Yan, Yinghan1; Ai, Nana4; Li, Jianjie1,2; Zhao, Ming1,2; Rajendran, Barani Kumar1,2; Liu, Jianlin1,2; Shao, Fangyuan1,2; Sun, Heng1,2,3; Choi, Tak Kan1,2; Zhu, Wenli5; Luo, Guanghui5; Liu, Shuiming5; Li Xu, De5; Chan, Kin Long5; Zhao, Qi1,2,3; Miao, Kai1,2,3; Luo, Kathy Qian1,2,3; Ge, Wei3,4; Xu, Xiaoling1,2,3; Wang, Guanyu6,7; Liu, Tzu-Ming1,2,3; Deng, Chu-Xia1,2,3

2021

10.7150/thno.59533

Theranostics   影响因子和分区

1838-7640

["The feasibility of personalized medicine for cancer treatment is largely hampered by costly, labor-intensive and time-consuming models for drug discovery. Herein, establishing new pre-clinical models to tackle these issues for personalized medicine is urgently demanded.","Methods: We established a three-dimensional tumor slice culture (3D-TSC) platform incorporating label-free techniques for time-course experiments to predict anti-cancer drug efficacy and validated the 3D-TSC model by multiphoton fluorescence microscopy, RNA sequence analysis, histochemical and histological analysis.","Results: Using time-lapse imaging of the apoptotic reporter sensor C3 (C3), we performed cell-based high-throughput drug screening and shortlisted high-efficacy drugs to screen murine and human 3D-TSCs, which validate effective candidates within 7 days of surgery. Histological and RNA sequence analyses demonstrated that 3D-TSCs accurately preserved immune components of the original tumor, which enables the successful achievement of immune checkpoint blockade assays with antibodies against PD-1 and/or PD-LI . Label-free multiphoton fluorescence imaging revealed that 3D-TSCs exhibit lipofuscin autofluorescence features in the time-course monitoring of drug response and efficacy.","Conclusion: This technology accelerates precision anti-cancer therapy by providing a cheap, fast, and easy platform for anti-cancer drug discovery."]

3D tumor slice culture apoptosis FRET technique label-free personalized medicine

SCI

英语

通讯

University of Macau["CPG 2021-00021-FHS","MYRG 2017-00113-FHS","MYRG 2018-00070-FHS","MYRG2019-00067-FHS"] ; Science and Technology Development Fund, Macau SAR["094/2015/A3","122/2016/A3","018/2017/A1","0011/2019/AKP","0112/2019/A2","0034/2019/AGJ","048/2019/A1"] ; Natural Science Foundation of China[32070681,82030094]

Research & Experimental Medicine

Medicine, Research & Experimental

WOS:000705513100002

IVYSPRING INT PUBL

Web of Science

1.Univ Macau, Fac Hlth Sci, Ctr Canc, Macau, Peoples R China
2.Univ Macau, Fac Hlth Sci, Ctr Precis Med Res & Training, Macau, Peoples R China
3.Univ Macau, MOE Frontier Sci Ctr Precis Oncol, Macau, Peoples R China
4.Univ Macau, Fac Hlth Sci, Ctr Reprod Dev & Aging, Macau, Peoples R China
5.Kiang Wu Hosp, Macau, Peoples R China
6.Southern Univ Sci & Technol, Dept Biol, Sch Life Sci, Shenzhen, Peoples R China
7.Southern Univ Sci & Technol, Guangdong Prov Key Lab Computat Sci & Mat Design, Shenzhen, Peoples R China

Xing, Fuqiang,Liu, Yu-Cheng,Huang, Shigao,et al. Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform[J]. Theranostics,2021,11(19).

Xing, Fuqiang.,Liu, Yu-Cheng.,Huang, Shigao.,Lyu, Xueying.,Su, Sek Man.,...&Deng, Chu-Xia.(2021).Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform.Theranostics,11(19).

Xing, Fuqiang,et al."Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform".Theranostics 11.19(2021).