南方科技大学知识苑(SUSTech KC): Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole-genome sequencing

题名	Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole-genome sequencing
作者	Bi，Jing 1; Guo，Qinglong1 ; Fu，Xiangdong 1; Liang，Juan 2; Zeng，Lidong 3; Ou，Min 1; Zhang，Juanjuan 1; Wang，Zhaoqin 1; Sun，Yicheng 4; Liu，Lei1 ; Zhang，Guoliang 1
通讯作者	Zhang，Guoliang
发表日期	2021-11-01
DOI	10.1016/j.ijid.2021.09.028
发表期刊	INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES 影响因子和分区
ISSN	1201-9712
EISSN	1878-3511
卷号	112页码:189-194
摘要	Objectives: Gatifloxacin (GAT), a fourth-generation fluoroquinolone (FQ), is used to treat drug-resistant tuberculosis. Although DNA gyrase mutations are the leading cause of FQ resistance, mutations conferring resistance to GAT remain inadequately characterized. Methods: GAT-resistant mutants were selected from 7H10 agar plates containing 0.5 mg/L GAT (critical concentration). Mutations involved in GAT resistance were identified through whole-genome sequencing. Results: In total, 123 isolates demonstrated resistance to GAT. Among these isolates, 55.3% (68/123) had gyrA gene mutations [G280A (D94N), A281G (D94G), G280T (D94Y) and G262T (G88C)]. The remainder (44.7%, 55/123) harboured gyrB gene mutations [A1495G (N499D), C1497A (N499K), C1497G (N499K) and A1503C (E501D)]. Conclusions: Mutations in the gyrA and gyrB genes are the main mechanisms of GAT resistance. These findings provide new insight into GAT resistance, and contribute to molecular diagnosis of GAT resistance in the clinical setting.
关键词	Fluoroquinolones Gatifloxacinresistance gyrA (Rv0005) gyrB (Rv0006) Mycobacterium tuberculosis
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Key Research and Development Plan["2020YFA0907200","2019YFC0840602"] ; Natural Science Foundation of China[81873958,81802058] ; Guangdong Foundation for Basic and Applied Basic Research[2019B1515120041] ; Guangdong Scientific and Technolog-ical Foundation[2020B1111170014] ; Shenzhen Scientific and Technological Foundation["KCXFZ202002011007083","JCYJ20180228162321234"] ; Sanming Project of Medicine in Shen-zhen[SZSM201911009] ; China Postdoctoral Science Foundation[2020M670085ZX]
WOS研究方向	Infectious Diseases
WOS类目	Infectious Diseases
WOS记录号	WOS:000711042800003
出版者	ELSEVIER SCI LTD
ESI学科分类	IMMUNOLOGY
Scopus记录号	2-s2.0-85117157133
来源库	Scopus
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/254224
专题	南方科技大学第二附属医院
作者单位	1.National Clinical Research Center for Infectious Diseases,Guangdong Provincial Clinical Research Center for Tuberculosis,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,China 2.Biomedical Translational Research Institute,Faculty of Medical Science,Jinan University,Guangzhou,510632,China 3.GeneMind Biosciences Co. Ltd,Shenzhen,China 4.Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,China
第一作者单位	南方科技大学第二附属医院
通讯作者单位	南方科技大学第二附属医院
第一作者的第一单位	南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Bi，Jing,Guo，Qinglong,Fu，Xiangdong,et al. Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole-genome sequencing[J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES,2021,112:189-194.
APA	Bi，Jing.,Guo，Qinglong.,Fu，Xiangdong.,Liang，Juan.,Zeng，Lidong.,...&Zhang，Guoliang.(2021).Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole-genome sequencing.INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES,112,189-194.
MLA	Bi，Jing,et al."Characterizing the gene mutations associated with resistance to gatifloxacin in Mycobacterium tuberculosis through whole-genome sequencing".INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES 112(2021):189-194.