南方科技大学知识苑(SUSTech KC): RpoB mutations and effects on rifampin resistance in mycobacterium tuberculosis

题名	RpoB mutations and effects on rifampin resistance in mycobacterium tuberculosis
作者	Li，Ma Chao 1; Lu，Jie 2; Lu，Yao 1,3; Xiao，Tong Yang 4; Liu，Hai Can 1; Lin，Shi Qiang 5; Xu，Da 1; Li，Gui Lian 1; Zhao，Xiu Qin 1; Liu，Zhi Guang 1; Zhao，Li Li 1; Wan，Kang Lin 1
发表日期	2021
DOI	10.2147/IDR.S333433
发表期刊	Infection and Drug Resistance 影响因子和分区
EISSN	1178-6973
卷号	14页码:4119-4128
摘要	Objective: To investigate the mutations within the whole rpoB gene of Mycobacterium tuberculosis and analyze their effects on rifampin (RIF) resistance based on crystal structure. Methods: We sequenced the entire rpoB gene in 175 tuberculosis isolates and quantified their minimum inhibitory concentrations using microplate-based assays. Additionally, the structural interactions between wild-type/mutant RpoB and RIF were also analyzed. Results: Results revealed that a total of 34 mutations distributed across 17 different sites within the whole rpoB gene were identified. Of the 34 mutations, 25 could alter the structural interaction between RpoB and RIF and contribute to RIF resistance. Statistical analysis showed that S450L, H445D, H445Y and H445R mutations were associated with high-level RIF resistance, while D435V was associated with moderate-level RIF resistance. Conclusion: Some mutations within the rpoB gene could affect the interaction between RpoB and RIF and thus are associated with RIF resistance. These findings could be helpful to design new antibiotics and develop novel diagnostic tools for drug resistance in TB.
关键词	Mutation Mycobacterium tuberculosis Rifampin resistance Structure
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
WOS记录号	WOS:000707363400005
Scopus记录号	2-s2.0-85117243915
来源库	Scopus
引用统计	被引频次[WOS]：31
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/254272
专题	南方科技大学第二附属医院
作者单位	1.State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China 2.Beijing Key Laboratory for Pediatric Diseases of Otolaryngology,Head and Neck Surgery,MOE Key Laboratory of Major Diseases in Children,Beijing Pediatric Research Institute,Beijing Children’s Hospital,Capital Medical University,National Center for Children’s Health,Beijing,China 3.School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China 4.Guangdong Key Laboratory for Diagnosis & Treatment of Emerging Infectious Diseases,Shenzhen Third People’s Hospital,Southern University of Science and Technology,Shenzhen,China 5.Department of Bioinformatics,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,China
推荐引用方式 GB/T 7714	Li，Ma Chao,Lu，Jie,Lu，Yao,et al. RpoB mutations and effects on rifampin resistance in mycobacterium tuberculosis[J]. Infection and Drug Resistance,2021,14:4119-4128.
APA	Li，Ma Chao.,Lu，Jie.,Lu，Yao.,Xiao，Tong Yang.,Liu，Hai Can.,...&Wan，Kang Lin.(2021).RpoB mutations and effects on rifampin resistance in mycobacterium tuberculosis.Infection and Drug Resistance,14,4119-4128.
MLA	Li，Ma Chao,et al."RpoB mutations and effects on rifampin resistance in mycobacterium tuberculosis".Infection and Drug Resistance 14(2021):4119-4128.