iTRAQ-based proteomic analysis of human umbilical vein endothelial cells with platelet endothelial aggregation receptor-1 knockdown

Yue, Yongjian1,2; Liu, Shengguo1,2; Han, Xuemei1,2; Wang, Minlian1,2; Li, Yazhen1,2; Huang, Qijun1,2; Li, Bo2,3; Yang, Mo4; Dai, Yong2,5; Fu, Yingyun1,2

2019-08

10.1002/jcb.28494

JOURNAL OF CELLULAR BIOCHEMISTRY   影响因子和分区

0730-2312

1097-4644

The disorders of hemostasis and coagulation were believed to be the main contributors to the pathogenesis of pulmonary thromboembolism (PTE), and platelets are the basic factors regulating hemostasis and coagulation and play important roles in the process of thrombosis. This study investigated the proteome of human umbilical vein endothelial cells (HUVECs) with platelet endothelial aggregation receptor-1 (PEAR1) knockdown using the isobaric tags for relative and absolute quantitation (iTRAQ) method and analyzed the role of differential abundance proteins (DAPs) in the regulation of platelets aggregation. Our results showed that the conditioned media-culturing HUVECs with PEAR1 knockdown partially suppressed the adenosine diphosphate (ADP)-induced platelet aggregation. The proteomics analysis was performed by using the iTRAQ technique, and a total of 215 DAPs (124 protein was upregulated and 91 protein were downregulated) were identified. The Gene Ontology (GO) enrichment analysis showed that proteins related to platelet alpha granule, adenosine triphosphate metabolic process, and endocytosis were significantly enriched. Further, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also identified the significant enrichment of endocytosis-related pathways. The real-time polymerase chain reaction assay confirmed that the expression of P2Y(12), mitochondrial carrier 2, NADH dehydrogenase (ubiquinone) iron-sulfur protein 3, and ubiquinol-cytochrome c reductase hinge protein are significantly downregulated in the HUVECs with PEAR1 knockdown. In conclusion, our in vitro results implicated that DAPs induced by PEAR1 knockdown might contribute to the platelet aggregation. Proteomic studies by employing GO enrichment and KEGG pathway analysis suggested that the potential effects of DAPs on platelet aggregation may be linked to the balance of ADP synthesis or degradation in mitochondria.

differential abundance proteins endocytosis differential abundance proteins receptor-1 platelets aggregation pulmonary thromboembolism

SCI

英语

第一 ; 通讯

National Key Research and Development Program of China[2016YFC1304400]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000471718300022

WILEY

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Key Lab Shenzhen Resp Dis, Dept Pulm & Crit Care Med,Shenzhen Inst Resp Dis, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Dept Pediat, Shenzhen, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Affiliated Hosp 7, Shenzhen, Guangdong, Peoples R China
5.Southern Univ Sci & Technol, Affiliated Hosp 1, Clin Med Res Ctr, Shenzhen, Guangdong, Peoples R China

Yue, Yongjian,Liu, Shengguo,Han, Xuemei,et al. iTRAQ-based proteomic analysis of human umbilical vein endothelial cells with platelet endothelial aggregation receptor-1 knockdown[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2019,120(8):12300-12310.

Yue, Yongjian.,Liu, Shengguo.,Han, Xuemei.,Wang, Minlian.,Li, Yazhen.,...&Fu, Yingyun.(2019).iTRAQ-based proteomic analysis of human umbilical vein endothelial cells with platelet endothelial aggregation receptor-1 knockdown.JOURNAL OF CELLULAR BIOCHEMISTRY,120(8),12300-12310.

Yue, Yongjian,et al."iTRAQ-based proteomic analysis of human umbilical vein endothelial cells with platelet endothelial aggregation receptor-1 knockdown".JOURNAL OF CELLULAR BIOCHEMISTRY 120.8(2019):12300-12310.