Wearable optical resolution photoacoustic microscopy

Chen, Qian1,2; Xie, Huikai3; Xi, Lei1

2019-08

10.1002/jbio.201900066

Journal of Biophotonics   影响因子和分区

1864-063X

1864-0648

Optical resolution photoacoustic microscopy (ORPAM) is an emerging imaging technique, which has been extensively used to study various brain activities and disorders of the anesthetized/restricted rodents with a special focus on the morphological and functional visualization of cerebral cortex. However, it is challenging to develop a wearable photoacoustic microscope, which enables the investigation of brain activities/disorders on freely moving rodents. Here, we report a wearable and robust optical resolution photoacoustic microscope (W-ORPAM), which utilizes a small, light, stable and fast optical scanner. This wearable imaging probe features high spatiotemporal resolution, large field of view (FOV) and easy assembly as well as adjustable optical focus during the in vivo experiment, which makes it accessible to image cerebral cortex activities of freely moving rodents. To demonstrate the advantages of this technique, we used W-ORPAM to monitor both morphological and functional variations of vasculature in cerebral cortex during the induction of ischemia and reperfusion of a freely moving rat.

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[81571722] ; National Natural Science Foundation of China[61775028] ; National Natural Science Foundation of China[61528401]

Biochemistry & Molecular Biology ; Biophysics ; Optics

Biochemical Research Methods ; Biophysics ; Optics

WOS:000480614900005

WILEY-V C H VERLAG GMBH

20191906900957

Acoustic microscopes ; Brain ; Laser recording ; Mammals ; Neurophysiology ; Scanning ; Wearable technology

Biomedical Engineering:461.1 ; Medicine and Pharmacology:461.6 ; Laser Applications:744.9 ; Imaging Techniques:746 ; Acoustic Devices:752.1

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Elect Sci & Technol China, Sch Elect Sci & Engn, Chengdu, Sichuan, Peoples R China
3.Univ Florida, Dept Elect & Comp Engn, Gainesville, FL USA

Chen, Qian,Xie, Huikai,Xi, Lei. Wearable optical resolution photoacoustic microscopy[J]. Journal of Biophotonics,2019,12(8).

Chen, Qian,Xie, Huikai,&Xi, Lei.(2019).Wearable optical resolution photoacoustic microscopy.Journal of Biophotonics,12(8).

Chen, Qian,et al."Wearable optical resolution photoacoustic microscopy".Journal of Biophotonics 12.8(2019).