A comprehensive analysis of transcriptome and phenolic compound profiles suggests the role of flavonoids in cotyledon greening in Catharanthus roseus seedling

Yu, Bofan1,2; Pan, Yajie2,3; Liu, Yang1,2; Chen, Qi2; Guo, Xiaorui1,2; Tang, Zhonghua1,2

2021-10-01

10.1016/j.plaphy.2021.07.028

PLANT PHYSIOLOGY AND BIOCHEMISTRY   影响因子和分区

0981-9428

1873-2690

During seedling photo-morphogenesis, cotyledon greening is a vital developmental process and a moment of responding to light stress. An increasing number of reports suggest the function of natural antioxidant protection of phenolic compounds in plant growth and development processes. Due to the antioxidant functions, flavonoids allow plants to respond to abiotic or biotic stresses. As one of the plants rich in secondary metabolites, Catharanthus roseus has drawn great academic interest due to its richness of diverse secondary metabolites with medicinal values. To assess the distribution and function of phenolic compounds during cotyledon greening, combined phenolic profiling and transcriptome were applied in C. roseus seedling through ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometer (UPLC-Q-TOF/MS) and high throughput RNA sequencing, respectively. Results herein showed that light-exposed greening cotyledon accumulated large amounts of C6C3C6-type flavonoids, suggesting the function in repressing reactive oxygen species (ROS) generation to improve light adaptation and seedling survival. Moreover, synergistic up-regulation of relevant genes involved in flavonoids pathway, including PAL, C4H, CHS, FLS, and F3'H, was monitored in response to light. Several crucial candidate transcription factors including bHLH, MYB, and B-box families were likely to function, and thereinto, CrHY5 (CRO_T122304) and CRO_T137938 revealed a prompt response to light, supposing to induce flavonoids accumulation by targeting CHS and FLS. Therefore, this study provided new insight into the potential regulation and underlying roles of flavonoids to improve light acclimation during cotyledon greening.

Catharanthus roseus Cotyledon greening Reactive oxygen species Phenolic compounds RNA-seq analysis Transcription factors

SCI

英语

其他

Fundamental Research Funds for the Central Universities[2572020DY04]

Plant Sciences

Plant Sciences

WOS:000703775900018

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

PLANT & ANIMAL SCIENCE

Web of Science

1.Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Harbin 150040, Peoples R China
2.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
3.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Peoples R China

Yu, Bofan,Pan, Yajie,Liu, Yang,et al. A comprehensive analysis of transcriptome and phenolic compound profiles suggests the role of flavonoids in cotyledon greening in Catharanthus roseus seedling[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2021,167.

Yu, Bofan,Pan, Yajie,Liu, Yang,Chen, Qi,Guo, Xiaorui,&Tang, Zhonghua.(2021).A comprehensive analysis of transcriptome and phenolic compound profiles suggests the role of flavonoids in cotyledon greening in Catharanthus roseus seedling.PLANT PHYSIOLOGY AND BIOCHEMISTRY,167.

Yu, Bofan,et al."A comprehensive analysis of transcriptome and phenolic compound profiles suggests the role of flavonoids in cotyledon greening in Catharanthus roseus seedling".PLANT PHYSIOLOGY AND BIOCHEMISTRY 167(2021).