南方科技大学知识苑(SUSTech KC): Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils in Vitro

题名	Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils in Vitro
作者	Wang，Gang 1; Guan，Jun 1; Li，Guojun 2; Wu，Fengtian 1; Yang，Qin 1; Huang，Chunhong 1; Shao，Junwei 1; Xu，Lichen 1; Guo，Zixuan 1; Zhou，Qihui 1; Zhu，Haihong 1; Chen，Zhi 1
通讯作者	Zhu，Haihong; Chen，Zhi
发表日期	2021
DOI	10.1155/2021/6803510
发表期刊	DISEASE MARKERS 影响因子和分区
ISSN	0278-0240
EISSN	1875-8630
卷号	2021
摘要	Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment of immune cells to the lungs exacerbates this process, although much remains unknown about the pathogenesis of COVID-19. Our study of lung type II pneumocyte cells (A549) demonstrated that ORF7, an open reading frame (ORF) in the genome of SARS-CoV-2, induced the production of CCL2, a chemokine that promotes the chemotaxis of monocytes, and decreased the expression of IL-8, a chemokine that recruits neutrophils. A549 cells also had an increased level of IL-6. The results of our chemotaxis Transwell assay suggested that ORF7 augmented monocyte infiltration and reduced the number of neutrophils. We conclude that the ORF7 of SARS-CoV-2 may have specific effects on the immunological changes in tissues after infection. These results suggest that the functions of other ORFs of SARS-CoV-2 should also be comprehensively examined.
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	National Science and Technology Major Project of China["2018ZX10302206003","2017ZX10202203-007","2017ZX10202203-008"] ; Zhejiang Health and Family Planning Research Projects["2016KYA162","2015KYA046"]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine ; Pathology
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental ; Pathology
WOS记录号	WOS:000810790100002
出版者	HINDAWI LTD
ESI学科分类	CLINICAL MEDICINE
Scopus记录号	2-s2.0-85117528502
来源库	Scopus
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/254532
专题	南方科技大学医学院南方科技大学第二附属医院
作者单位	1.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China 2.Department of Liver Diseases,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518000,China
推荐引用方式 GB/T 7714	Wang，Gang,Guan，Jun,Li，Guojun,et al. Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils in Vitro[J]. DISEASE MARKERS,2021,2021.
APA	Wang，Gang.,Guan，Jun.,Li，Guojun.,Wu，Fengtian.,Yang，Qin.,...&Chen，Zhi.(2021).Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils in Vitro.DISEASE MARKERS,2021.
MLA	Wang，Gang,et al."Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils in Vitro".DISEASE MARKERS 2021(2021).