Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells

Xia, Shumin1; Lim, Ying Bena2; Zhang, Zhen1; Wang, Yilin3; Zhang, Shan4; Lim, Chwee Teck1,2,5; Yim, Evelyn K. F.6; Kanchanawong, Pakorn1,2

2019-07-30

10.1016/j.celrep.2019.06.089

Cell Reports   影响因子和分区

2211-1247

Mechanical cues influence pluripotent stem cell differentiation, but the underlying mechanisms are not well understood. Mouse embryonic stem cells (mESCs) exhibit unusual cytomechanical properties, including low cell stiffness and attenuated responses to substrate rigidity, but the underlying structural basis remains obscure. Using super-resolution microscopy to investigate the actin cytoskeleton in mESCs, we observed that the actin cortex consists of a distinctively sparse and isotropic network. Surprisingly, the architecture and mechanics of the mESC actin cortex appear to be largely myosin II-independent. The network density can be modulated by perturbing Arp2/3 and formin, whereas capping protein (CP) negatively regulates cell stiffness. Transient Arp2/3-containing aster-like structures are implicated in the organization and mechanical homeostasis of the cortical network. By generating a low-density network that physically excludes myosin II, the interplay between Arp2/3, formin, and CP governs the nanoscale architecture of the actin cortex and prescribes the cytomechanical properties of mESCs.

SCI

英语

其他

Ministry of Education Academic Research Fund Tier 2[MOE-T2-1-124] ; Ministry of Education Academic Research Fund Tier 2[MOE-T2-1-045]

Cell Biology

Cell Biology

WOS:000477811200013

CELL PRESS

Web of Science

1.Natl Univ Singapore, Mechanobiol Inst, Singapore 117411, Singapore
2.Natl Univ Singapore, Dept Biomed Engn, Singapore 117583, Singapore
3.South Univ Sci & Technol China, Dept Biol, Shenzhen 518055, Peoples R China
4.Natl Univ Singapore, Dept Biol Sci, Singapore 117558, Singapore
5.Natl Univ Singapore, Inst Hlth Innovat & Technol iHealthtech, Singapore 117599, Singapore
6.Univ Waterloo, Dept Chem Engn, Waterloo, ON N2L 3G1, Canada

Xia, Shumin,Lim, Ying Bena,Zhang, Zhen,et al. Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells[J]. Cell Reports,2019,28(5):1251-+.

Xia, Shumin.,Lim, Ying Bena.,Zhang, Zhen.,Wang, Yilin.,Zhang, Shan.,...&Kanchanawong, Pakorn.(2019).Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.Cell Reports,28(5),1251-+.

Xia, Shumin,et al."Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells".Cell Reports 28.5(2019):1251-+.