Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples

Chen, Yu1,2; Wu, Peng3; Fu, Liang4; Liu, Yu-hong5; Zhang, Yao6; Zhao, Yanping3

2019-07-30

10.1038/s41598-019-47358-3

Scientific Reports   影响因子和分区

2045-2322

Genitourinary tuberculosis (GUTB) accounts for up to 40% of extrapulmonary tuberculosis cases. Rapid tests for GUTB are urgently needed because it is often associated with delayed health-care seeking, leading to serious consequences. This study evaluated the performance of the Xpert MTB/RIF assay in the rapid diagnosis of urinary tract tuberculosis (UTB) and rifampicin-resistant tuberculosis with urine specimens. In all, 302 patients were included from four hospitals in China. Suspected UTB patients were tested with Xpert, smear, and MGIT 960 culture. Drug susceptibility testing (DST) was conducted for culture-positive cases. The performance of the assays was evaluated against MGIT 960 culture and a composite reference standard (CRS). Among all participants, 150 (49.7%) had CRS-positive UTB, of whom 36 (24.0%) were culture-confirmed. Against culture, Xpert and smear achieved a sensitivity of 94.4% (95% CI: 81.3-99.3%) and 22.2% (95% CI: 10.1-39.2%), respectively. Against CRS, the sensitivity of Xpert, smear and culture was 41.3% (95% CI: 33.4-49.7%), 7.3% (95% CI: 3.7-12.7%), and 24.0% (95% CI: 17.4-31.6%). Xpert had better performance than smear and culture in detecting UTB from urine samples and could be considered for the diagnosis of UTB. Moreover, Xpert showed better performance than MGIT 960-based DST using urine culture.

SCI

英语

其他

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000477858900029

NATURE PUBLISHING GROUP

Web of Science

1.Shenyang Tenth Peoples Hosp, Shenyang 110044, Liaoning, Peoples R China
2.Shenyang Chest Hosp, Dept TB, Shenyang 110044, Liaoning, Peoples R China
3.Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, Pokfulam, 1-F,Patrick Manson Bldg North,7 Sassoon Rd, Hong Kong, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Pulm Dis Dept 2, Shenzhen 518112, Guangdong, Peoples R China
5.China TB Clin Trial Consortium, Beijing 101149, Peoples R China
6.Family Hlth Int 360, Global Res Serv, Beijing 101149, Peoples R China

Chen, Yu,Wu, Peng,Fu, Liang,et al. Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples[J]. Scientific Reports,2019,9.

Chen, Yu,Wu, Peng,Fu, Liang,Liu, Yu-hong,Zhang, Yao,&Zhao, Yanping.(2019).Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples.Scientific Reports,9.

Chen, Yu,et al."Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples".Scientific Reports 9(2019).