Enabling peristalsis of human colon tumor organoids on microfluidic chips

Fang, Guocheng1; Lu, Hongxu1; Al-Nakashli, Russul2; Chapman, Robert3; Zhang, Yingqi4; Ju, Lining Arnold4; Lin, Gungun1; Stenzel, Martina H.2; Jin, Dayong1,5

2022

10.1088/1758-5090/ac2ef9

Biofabrication   影响因子和分区

1758-5082

1758-5090

Peristalsis in the digestive tract is crucial to maintain physiological functions. It remains challenging to mimic the peristaltic microenvironment in gastrointestinal organoid culture. Here, we present a method to model the peristalsis for human colon tumor organoids on a microfluidic chip. The chip contains hundreds of lateral microwells and a surrounding pressure channel. Human colon tumor organoids growing in the microwell were cyclically contracted by pressure channel, mimicking the in vivo mechano-stimulus by intestinal muscles. The chip allows the control of peristalsis amplitude and rhythm and the high throughput culture of organoids simultaneously. By applying 8% amplitude with 8 similar to 10 times min(-1), we observed the enhanced expression of Lgr5 and Ki67. Moreover, ellipticine-loaded polymeric micelles showed reduced uptake in the organoids under peristalsis and resulted in compromised anti-tumor efficacy. The results indicate the importance of mechanical stimuli mimicking the physiological environment when using in vitro models to evaluate nanoparticles. This work provides a method for attaining more reliable and representative organoids models in nanomedicine.

colon tumor organoids peristalsis microfluidic chip micelle nano drug carriers

SCI ; EI

英语

通讯

China Scholarship Council[201708140082] ; National Health and Medical Research Council (NHMRC)[GNT1160635] ; ARC Industry Transformational Research Hub Scheme[IH150100028] ; ARC Linkage Infrastructure, Equipment and Facilities (LIEF) Project[LE180100043] ; Australian Government["ACSRF65827","2017YFE0132300"] ; Chinese Government["ACSRF65827","2017YFE0132300"]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000710694400001

IOP Publishing Ltd

20214711185352

Drug delivery ; Fluidic devices ; Medical nanotechnology ; Microfluidics ; Physiological models ; Physiology ; Tumors

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Hydraulic Equipment and Machinery:632.2 ; Microfluidics:632.5.1 ; Control Equipment:732.1 ; Nanotechnology:761 ; Colloid Chemistry:801.3

Web of Science

1.Univ Technol Sydney, Sch Math & Phys Sci, Inst Biomed Mat & Devices, Broadway Ultimo, Sydney, NSW 2007, Australia
2.Univ New South Wales, Sch Chem, Sydney, NSW 2052, Australia
3.Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
4.Univ Sydney, Fac Engn, Sch Biomed Engn, Sydney, NSW 2008, Australia
5.Southern Univ Sci & Technol, UTS SUSTech Joint Res Ctr Biomed Mat & Devices, Dept Biomed Engn, Shenzhen, Peoples R China

Fang, Guocheng,Lu, Hongxu,Al-Nakashli, Russul,et al. Enabling peristalsis of human colon tumor organoids on microfluidic chips[J]. Biofabrication,2022,14(1).

Fang, Guocheng.,Lu, Hongxu.,Al-Nakashli, Russul.,Chapman, Robert.,Zhang, Yingqi.,...&Jin, Dayong.(2022).Enabling peristalsis of human colon tumor organoids on microfluidic chips.Biofabrication,14(1).

Fang, Guocheng,et al."Enabling peristalsis of human colon tumor organoids on microfluidic chips".Biofabrication 14.1(2022).