南方科技大学知识苑(SUSTech KC): Structure of Arabidopsis thaliana N-6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N-6-methyl-AMP recognition and processing

题名	Structure of Arabidopsis thaliana N-6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N-6-methyl-AMP recognition and processing
作者	Wu, Baixing; Zhang, Dong ; Nie, Hongbo; Shen, Senlin; Li, Yan; Li, Sisi
通讯作者	Wu, Baixing; Li, Sisi
发表日期	2019-07-19
DOI	10.1080/15476286.2019.1642712
发表期刊	RNA Biology 影响因子和分区
ISSN	1547-6286
EISSN	1555-8584
卷号	16 期号:10页码:1504-1512
摘要	Arabidopsis thaliana aminohydrolase (AtADAL) has been shown to be involved in the metabolism of N-6-methyl-AMP, a proposed intermediate during m(6)A-modified RNA metabolism, which can be subsequently incorporated into newly synthesized RNA by Pol II. It has been proposed that AtADAL will prevent N-6-methyl-AMP reuse and catabolize it to inosine monophosphate (IMP). Here, we have solved the crystal structures of AtADAL in the apo form and in complex with GMP and IMP in the presence of Zn2+. We have identified the substrate-binding pocket of AtADAL and compared it with that for adenosine deaminase (ADA), adenine deaminase (ADE) and AMP deaminase (AMPD) from multiple species. The comparisons reveal that plant ADAL1 may have the potential ability to catalyze different alkyl-group substituted substrates.
关键词	ADAL Arabidopsis thaliana N-6-methyl-AMP RNA metabolism crystal structure
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Shenzhen Sci-Tech Fund[KYTDPT20181011104005]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000478108900001
出版者	TAYLOR & FRANCIS INC
来源库	Web of Science
引用统计	被引频次[WOS]：6
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/25488
专题	生命科学学院_生物系
作者单位	Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
第一作者单位	生物系
通讯作者单位	生物系
第一作者的第一单位	生物系
推荐引用方式 GB/T 7714	Wu, Baixing,Zhang, Dong,Nie, Hongbo,et al. Structure of Arabidopsis thaliana N-6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N-6-methyl-AMP recognition and processing[J]. RNA Biology,2019,16(10):1504-1512.
APA	Wu, Baixing,Zhang, Dong,Nie, Hongbo,Shen, Senlin,Li, Yan,&Li, Sisi.(2019).Structure of Arabidopsis thaliana N-6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N-6-methyl-AMP recognition and processing.RNA Biology,16(10),1504-1512.
MLA	Wu, Baixing,et al."Structure of Arabidopsis thaliana N-6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N-6-methyl-AMP recognition and processing".RNA Biology 16.10(2019):1504-1512.