Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array

Yin, Jiaxiang1,2,3; Mou, Lei2; Yang, Mingzhu2; Zou, Wenwu1; Du, Chang1,3,4; Zhang, Wei2; Jiang, Xingyu2,5,6

2019-07-04

10.1016/j.aca.2019.01.054

ANALYTICA CHIMICA ACTA   影响因子和分区

0003-2670

1873-4324

This report demonstrates that a microfluidic device with integrated silicon filter exhibits outstanding capture efficiency and superior enrichment purity when employed to separate tumor cells from whole blood samples. We fabricate the silicon filter with pyramidal microcavity array (MCA) by micro-fabrication. We design the structure of the cavity to efficiently enrich tumor cells, while allowing hematologic cells to deform and pass through. The capture efficiency of MCF-7, SW620 and Hela cells spiked in 1 mL of whole blood are approximately 80%. Unwanted white blood cells (WBCs) trapped on the MCA are below 0.003%. In addition, this microfluidic device successfully identifies circulating tumor cells (CTCs) in 5 of 6 patients' blood samples, with a range of 5-86 CTCs per mL. These results reveal that the disposable microfluidic device can effectively enrich tumor cells with different sizes and various morphologies, while maintaining high capture efficiency and purity. Therefore, this label-free technique can serve as a versatile platform to facilitate CTCs analysis in diverse biochemical applications. (C) 2019 Elsevier B.V. All rights reserved.

CTCs Efficient capture Low background signals Pyramidal MCA

SCI ; EI

英语

通讯

[2017YFA0205901]

Chemistry

Chemistry, Analytical

WOS:000461632900014

ELSEVIER SCIENCE BV

20190806520838

Blood ; Efficiency ; Fluidic devices ; Microcavities ; Microfluidics ; Tumors

Biological Materials and Tissue Engineering:461.2 ; Hydraulic Equipment and Machinery:632.2 ; Microfluidics:632.5.1 ; Electronic Components and Tubes:714 ; Production Engineering:913.1

CHEMISTRY

Web of Science

1.South China Univ Technol, Sch Mat Sci & Engn, Dept Biomed Engn, Guangzhou 510641, Guangdong, Peoples R China
2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
3.South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China
4.Minist Educ, Key Lab Biomed Mat Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China
5.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
6.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China

Yin, Jiaxiang,Mou, Lei,Yang, Mingzhu,et al. Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array[J]. ANALYTICA CHIMICA ACTA,2019,1060:133-141.

Yin, Jiaxiang.,Mou, Lei.,Yang, Mingzhu.,Zou, Wenwu.,Du, Chang.,...&Jiang, Xingyu.(2019).Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array.ANALYTICA CHIMICA ACTA,1060,133-141.

Yin, Jiaxiang,et al."Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array".ANALYTICA CHIMICA ACTA 1060(2019):133-141.