南方科技大学知识苑(SUSTech KC): Evolution of Nucleic Acid Aptamers Capable of Specifically Targeting Glioma Stem Cells via Cell-SELEX

题名	Evolution of Nucleic Acid Aptamers Capable of Specifically Targeting Glioma Stem Cells via Cell-SELEX
作者	Wu, Qiaoyi 1; Lin, Ningqin 1; Tian, Tian 2; Zhu, Zhi 2; Wu, Liang 1; Wang, Hongyao 1; Wang, Dengliang 1; Kang, Dezhi 1; Tian, Ruijun3 ; Yang, Chaoyong 2,4
通讯作者	Kang, Dezhi; Tian, Ruijun; Yang, Chaoyong
发表日期	2019-07-02
DOI	10.1021/acs.analchem.8b05941
发表期刊	ANALYTICAL CHEMISTRY 影响因子和分区
ISSN	0003-2700
EISSN	1520-6882
卷号	91 期号:13页码:8070-8077
摘要	Glioma stem cells (GSCs), a particular group of cells from gliomas, are capable of infinite proliferation and differentiation. Recent studies have shown that GSCs may be the root of tumor recurrence, metastasis, and resistance. Early detection and targeted therapy of GSCs may significantly improve the survival rate of glioma patients. Therefore, molecular ligands capable of selectively recognizing GCSs are of great importance. The objective of this study is to generate DNA aptamers for selective identification of the molecular signature of GSCs using cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX). GSCs were used as the positive selection target, while U87 cells were used in negative cycles for removal of DNA molecules binding to common glioma cell lines. Finally, we successfully identified one aptamer named W5-7 with a K-d value of 4.9 +/- 1.4 nM. The sequence of the aptamer was further optimized, and its binding target was identified as a membrane protein. The aptamer W5-7 was stable in cerebral spinal fluid over 36 h and could also effectively detect glioma stem cells in cerebral spinal fluid samples. With its superb targeting properties and functional versatility, WS-7 holds great potential for use as a molecular probe for detecting and isolating GSCs.
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
重要成果	NI期刊
学校署名	通讯
资助项目	Program for Changjiang Scholars and Innovative Research Team in University[IRT13036]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000474477900015
出版者	AMER CHEMICAL SOC
EI入藏号	20193407339463
EI主题词	Antenna lobes ; Cell culture ; Cerebrospinal fluid ; Ligands ; Nucleic acids ; Patient treatment ; Stem cells
EI分类号	Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Physical Chemistry:801.4
ESI学科分类	CHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：23
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/25560
专题	理学院_化学系南方科技大学医学院生命科学学院_生物系
作者单位	1.Fujian Med Univ, Clin Med Coll 1, Affiliated Hosp 1, Dept Neurosurg,Dept Emergency Surg, Fuzhou 350004, Fujian, Peoples R China 2.Xiamen Univ, Coll Chem & Chem Engn,Dept Chem Biol, MOE,Key Lab Spectrochem Anal & Instrumentat, State Key Lab Phys Chem Solid Surfaces,Key Lab Ch, Xiamen 361005, Fujian, Peoples R China 3.Southern Univ Sci & Technol, Dept Chem, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen 518055, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200127, Peoples R China
通讯作者单位	化学系; 南方科技大学医学院
推荐引用方式 GB/T 7714	Wu, Qiaoyi,Lin, Ningqin,Tian, Tian,et al. Evolution of Nucleic Acid Aptamers Capable of Specifically Targeting Glioma Stem Cells via Cell-SELEX[J]. ANALYTICAL CHEMISTRY,2019,91(13):8070-8077.
APA	Wu, Qiaoyi.,Lin, Ningqin.,Tian, Tian.,Zhu, Zhi.,Wu, Liang.,...&Yang, Chaoyong.(2019).Evolution of Nucleic Acid Aptamers Capable of Specifically Targeting Glioma Stem Cells via Cell-SELEX.ANALYTICAL CHEMISTRY,91(13),8070-8077.
MLA	Wu, Qiaoyi,et al."Evolution of Nucleic Acid Aptamers Capable of Specifically Targeting Glioma Stem Cells via Cell-SELEX".ANALYTICAL CHEMISTRY 91.13(2019):8070-8077.