南方科技大学知识苑(SUSTech KC): PNIPAM-based extracellular K+ fluorescent sensor for high-throughput analysis

题名	PNIPAM-based extracellular K+ fluorescent sensor for high-throughput analysis
作者	Shen, Min; Pan, Tingting ; Ning, Juewei; Shi, Jiayan ; Liu, Hongtian ; Tian, Yanqing
通讯作者	Tian, Yanqing
发表日期	2021-12-01
DOI	10.1016/j.mtcomm.2021.102911
发表期刊	MATERIALS TODAY COMMUNICATIONS 影响因子和分区
EISSN	2352-4928
卷号	29
摘要	We report on the fabrication of K+ fluorescent sensor P2 based on thermoresponsive poly(N-isopropylacrylamide) and small molecule K+ fluorescent sensor KS. P2 changed its size with a range from 35 degrees C to 42 degrees C and exhibited lower critical solution temperature (LCST) at 38 degrees C. The LCST affected the polymer's nanostructures and microenvironment of KS, which in turn affected P2's fluorescence intensities and responses to K+. Furthermore, P2 exhibited high selectivity and sensitivity to K+ with a dynamic response range from 1 to 20 mM, enabling its suitableness for extracellular K+ analysis. The sensor was further used to in situ monitor the K+ fluctuation of Escherichia coli and B. Subtilis 168 bacteria under lysozyme stimulation in 96 well plate by a high throughput analysis approach, and cell species dependent K+ release was observed. The PNIPAM-based K+ sensor P2 shows potential application in antimicrobial drugs/peptides screening.
关键词	Potassium ion sensor Fluorescence PNIPAM High-throughput analysis
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China[21774054,21574061] ; Shenzhen Fundamental Research Programs[JCYJ20170412152922553]
WOS研究方向	Materials Science
WOS类目	Materials Science, Multidisciplinary
WOS记录号	WOS:000718040800002
出版者	ELSEVIER
EI入藏号	20220311463027
EI主题词	Acrylic monomers ; Amides ; Escherichia coli ; Potassium
EI分类号	Alkali Metals:549.1 ; Light/Optics:741.1 ; Organic Compounds:804.1
来源库	Web of Science
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/256189
专题	工学院_材料科学与工程系
作者单位	Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China
第一作者单位	材料科学与工程系
通讯作者单位	材料科学与工程系
第一作者的第一单位	材料科学与工程系
推荐引用方式 GB/T 7714	Shen, Min,Pan, Tingting,Ning, Juewei,et al. PNIPAM-based extracellular K+ fluorescent sensor for high-throughput analysis[J]. MATERIALS TODAY COMMUNICATIONS,2021,29.
APA	Shen, Min,Pan, Tingting,Ning, Juewei,Shi, Jiayan,Liu, Hongtian,&Tian, Yanqing.(2021).PNIPAM-based extracellular K+ fluorescent sensor for high-throughput analysis.MATERIALS TODAY COMMUNICATIONS,29.
MLA	Shen, Min,et al."PNIPAM-based extracellular K+ fluorescent sensor for high-throughput analysis".MATERIALS TODAY COMMUNICATIONS 29(2021).