Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers

Chen, Yumei1; Ma, Chiyu1,4; Liu, Lixiong2; He, Jingquan1; Zhu, Chengxin1; Zheng, Fengping1; Dai, Weier3; Hong, Xiaoping2; Liu, Dongzhou2; Tang, Donge1; Dai, Yong1,4

2021-10-31

Aging-US   影响因子和分区

1945-4589

Rheumatoid arthritis (RA) is an autoimmune disease described by joint destruction, synovitis and pannus formation. The gut microbiota acts as an environmental factor that plays an important role in RA, but little research regarding the etiopathogenic mechanisms of the microbiome in RA has been carried out. We used an integrated approach of 16S rRNA gene sequencing and ultrahigh-performance liquid chromatography-mass spectrometry-based metabolomics to analyze the structure and diversity of the intestinal flora and metabolites of the gut microbiota in RA patients compared with healthy subjects. In this study, a-diversity analysis of the gut microbiota showed that there was no significant difference between the healthy control (HC) and RA groups. However, beta-diversity analysis showed that there was a significant difference between the two groups. Further analysis of alteration of the gut microbiota revealed that at the phylum level, the relative abundance of p_Bacteroidetes was significantly decreased in the RA group, while that of Verrucomicrobia and Proteobacteria was significantly increased in the RA group. At the genus level, Bacteroides, Faecalibacterium and some probiotics were decreased in the RA group, while 97 genera, including Lactobacillus, Streptococcus and Akkermansia, were increased in the RA group. Seventy-four differentially abundant metabolites were identified between the HC and RA groups, and we identified two potential biomarkers (9,12-octadecadiynoic acid and 10Z-nonadecenoic acid) in RA.

rheumatoid arthritis gut microbiota metabolites biomarkers

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81671596] ; Science and Technology Planning Project of Guangdong Province, China[2017B020209001] ; National Science Foundation for Young Scientists of China[31700795] ; Key Research and Development Program of Guangdong Province[2019B020229001] ; group of Rheumatology and Immunology["SYJY201704","SYJY201705"]

Cell Biology ; Geriatrics & Gerontology

Cell Biology ; Geriatrics & Gerontology

WOS:000714427600017

IMPACT JOURNALS LLC

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ,Shenzhen Engn Res Ctr Autoimmune Dis, Shenzhen Peoples Hosp,Guangdong Prov Engn Res Ctr, Clin Med Res Ctr,Affiliated Hosp 1,Clin Med Coll, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Rheumatol & Immunol,Affiliated Hosp 1,Clin M, Shenzhen 518020, Guangdong, Peoples R China
3.Univ Texas Austin, Coll Nat Sci, Austin, TX 78721 USA
4.Guilin 924 Hosp, Guangxi Key Lab Metab Dis Res, Nanning 541002, Peoples R China

Chen, Yumei,Ma, Chiyu,Liu, Lixiong,et al. Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers[J]. Aging-US,2021,13(20).

Chen, Yumei.,Ma, Chiyu.,Liu, Lixiong.,He, Jingquan.,Zhu, Chengxin.,...&Dai, Yong.(2021).Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers.Aging-US,13(20).

Chen, Yumei,et al."Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers".Aging-US 13.20(2021).