Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing T cells and regulatory T cells

Li, Zhixia1,2; Shi, Xiajie1,2; Liu, Junbin1,2; Shao, Feng1,2; Huang, Gan1,2; Zhou, Zhiguang1,2; Zheng, Peilin1,2,3

2019-07

10.1096/fj.201900146R

FASEB JOURNAL   影响因子和分区

0892-6638

1530-6860

Type 1 diabetes (T1D) is an autoimmune disease characterized by the immune-mediated destruction of insulin-producing beta cells. Recent studies showed that in addition to malaria, artemisinin and its derivative, artesunate (AS), could alleviate several autoimmune diseases. However, whether AS has a role in the prevention or treatment of T1D is still unknown. Therefore, in this study we administrated AS or DMSO in the drinking water of nonobese diabetic (NOD) mice, a mouse model of T1D. We found that AS administration significantly prevented the incidence of T1D. The frequency of IL-4-producing CD4(+) single-positive T cells and CD8(+) T cells was significantly elevated, and IFN-gamma-producing T cells were reduced in the spleen and pancreatic lymph nodes. In the pancreas, the skewing to IL-4-producing T cells was also observed. In addition, more regulatory T cells were found in the pancreas. mRNA levels of proinflammatory cytokines, including TNF-alpha and IL-6, were decreased. In addition, AS administration promoted the functional maturity of beta cells in vitro. Our findings demonstrate that AS administration can prevent T1D in NOD mice mainly by reducing autoimmune T cells and increasing protective T cells. Our data constitute the first functional study of AS in T1D, which may provide a new rationale for future translational studies.-Li, Z., Shi, X., Liu, J., Shao, F., Huang, G., Zhou, Z., Zheng, P. Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing T cells and regulatory T cells.

Chinese traditional medicine autoimmunity beta cells

SCI

英语

通讯

Graduate Innovation Project of Central South University[502211703] ; Graduate Innovation Project of Central South University[2018zzts921]

Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology

Biochemistry & Molecular Biology ; Biology ; Cell Biology

WOS:000476234700036

FEDERATION AMER SOC EXP BIOL

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Second Xiangya Hosp, Dept Metab & Endocrinol, Changsha, Hunan, Peoples R China
2.Cent S Univ, Natl Clin Res Ctr Metab Dis, Key Lab Diabet Immunol, Minist Educ, Changsha, Hunan, Peoples R China
3.Jinan Univ, Dept Endocrinol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Southern Univ Sci & Technol,Cli, Shenzhen, Peoples R China

Li, Zhixia,Shi, Xiajie,Liu, Junbin,et al. Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing T cells and regulatory T cells[J]. FASEB JOURNAL,2019,33(7):8241-8248.

Li, Zhixia.,Shi, Xiajie.,Liu, Junbin.,Shao, Feng.,Huang, Gan.,...&Zheng, Peilin.(2019).Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing T cells and regulatory T cells.FASEB JOURNAL,33(7),8241-8248.

Li, Zhixia,et al."Artesunate prevents type 1 diabetes in NOD mice mainly by inducing protective IL-4-producing T cells and regulatory T cells".FASEB JOURNAL 33.7(2019):8241-8248.