The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis

Zhao，Nan1,2; Su，Xiao Min1; Liu，Zhang Wei1; Zhou，Jin Xing1; Su，Yin Na1; Cai，Xue Wei1; Chen，Ling3; Wu，Zhe3; He，Xin Jian1,2,4

2021

10.1111/nph.17836

NEW PHYTOLOGIST   影响因子和分区

0028-646X

1469-8137

FLOWERING LOCUS M (FLM) is a well-known MADS-box transcription factor that is required for preventing early flowering under low temperatures in Arabidopsis thaliana. Alternative splicing of FLM is involved in the regulation of temperature-responsive flowering. However, how the basic transcript level of FLM is regulated is largely unknown. Here, we conducted forward genetic screening and identified a previously uncharacterized flowering repressor gene, UBA2c. Genetic analyses indicated that UBA2c represses flowering at least by promoting FLM transcription. We further demonstrated that UBA2c directly binds to FLM chromatin and facilitates FLM transcription by inhibiting histone H3K27 trimethylation, a histone marker related to transcriptional repression. UBA2c encodes a protein containing two putative RNA recognition motifs (RRMs) and one prion-like domain (PrLD). We found that UBA2c forms speckles in the nucleus and that both the RRMs and PrLD are required not only for forming the nuclear speckles but also for the biological function of UBA2c. These results identify a previously unknown flowering repressor and provide insights into the regulation of flowering time.

Arabidopsis FLM flowering time histone transcription UBA2c

SCI

英语

其他

WOS:000718935700001

PLANT & ANIMAL SCIENCE

2-s2.0-85118992248

Scopus

1.National Institute of Biological Sciences,Beijing,102206,China
2.Graduate School of Peking Union Medical College,Beijing,100730,China
3.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
4.Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing,100084,China

Zhao，Nan,Su，Xiao Min,Liu，Zhang Wei,et al. The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis[J]. NEW PHYTOLOGIST,2021.

Zhao，Nan.,Su，Xiao Min.,Liu，Zhang Wei.,Zhou，Jin Xing.,Su，Yin Na.,...&He，Xin Jian.(2021).The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis.NEW PHYTOLOGIST.

Zhao，Nan,et al."The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis".NEW PHYTOLOGIST (2021).