The long intergenic non-protein coding RNA 472 (LINC00472) aggravates neuropathic pain through the microRNA-300/high mobility group box protein 1 axis: a study using the chronic constrictive injury rat model

Zhang, Juan1; Liu, Jiao2; Ling, Diyang1; Zhao, Yan1; Wang, Zheyin1

2021-10-01

10.21037/apm-21-2651

Annals of Palliative Medicine   影响因子和分区

2224-5820

2224-5839

["Background: This study investigated the role and molecular mechanisms of the long intergenic nonprotein coding RNA 472 (LINC00472) in neuropathic pain using a chronic constrictive injury (CCI) rat model.","Methods: CCI rat model was established and PC12 cells were induced by LPS to simulate neuropathological injury in vivo and in vitro. The levels of LINC00472, miR-300, and high mobility group box protein 1 (HMGB1) in the spinal cord tissue of CCI rats and PC12 pheochromocytoma cells were assessed by qRT-PCR and western blot. The effects of LINC00472 on neuropathic pain in the CCI rats were observed by their pain behavior. ELISA was used to detect the levels of inflammatory cytokines in rat tissues and cells. The molecular mechanisms of LINC00472 were verified by luciferase experiments, RNA immunoprecipitation, and RNA pull down assays.","Results: The expression of LINC00472 and HMGB1 were upregulated, and the expression of miR-300 was downregulated in the spinal cord tissues of CCI rats and in PC12 cells. The upregulation of LINC00472 in CCI rats significantly induced the occurrence of neuropathic pain. In addition, downregulation of LINC00472 inhibited the inflammatory response of CCI rats and PC12 cells. This study identified miR-300 as a target gene of LINC00472, and HMGB1 as the target gene of miR-300. Further experiments confirmed that the expression of anti-miR-300 could partially reverse the anti-inflammatory effects and the reduction of neuropathic pain induced by low expression of LINC00472.","Conclusions: LINC00472 promotes the progression of neuropathic pain by reducing miR-300 expression and increasing HMGB1 expression. The LINC00472/miR-300/HMGB1 axis may be a novel therapeutic target for neuropathic pain."]

LINC00472 neuropathic pain inflammation miR-300 high mobility group box protein 1 (HMGB1)

SCI

英语

第一 ; 通讯

Health Care Sciences & Services

Health Care Sciences & Services

WOS:000720323100001

AME PUBL CO

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Pain Med, Shenzhen 518020, Peoples R China
2.Nanjing Med Univ, Affiliated Wuxi Peoples Hosp 2, Dept Anesthesiol, Wuxi, Jiangsu, Peoples R China

Zhang, Juan,Liu, Jiao,Ling, Diyang,et al. The long intergenic non-protein coding RNA 472 (LINC00472) aggravates neuropathic pain through the microRNA-300/high mobility group box protein 1 axis: a study using the chronic constrictive injury rat model[J]. Annals of Palliative Medicine,2021,10(11).

Zhang, Juan,Liu, Jiao,Ling, Diyang,Zhao, Yan,&Wang, Zheyin.(2021).The long intergenic non-protein coding RNA 472 (LINC00472) aggravates neuropathic pain through the microRNA-300/high mobility group box protein 1 axis: a study using the chronic constrictive injury rat model.Annals of Palliative Medicine,10(11).

Zhang, Juan,et al."The long intergenic non-protein coding RNA 472 (LINC00472) aggravates neuropathic pain through the microRNA-300/high mobility group box protein 1 axis: a study using the chronic constrictive injury rat model".Annals of Palliative Medicine 10.11(2021).