南方科技大学知识苑(SUSTech KC): A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein

题名	A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein
作者	Deng，Mengying 1; Yuan，Jing 2; Yang，Haibin3,4 ; Wu，Xuli 5; Wei，Xiaoyuan 1; Du，Yang 6; Wong，Garry 3; Tao，Yuyong 7; Liu，Gang 8; Jin，Zongwen 1; Chu，Jun 1
通讯作者	Jin，Zongwen; Chu，Jun
发表日期	2021
DOI	10.1021/acsnano.1c05164
发表期刊	ACS Nano 影响因子和分区
ISSN	1936-0851
EISSN	1936-086X
卷号	15 期号:11页码:17602-17612
摘要	A method for fast and highly sensitive detection of antibodies in serum would greatly facilitate the early diagnosis of disease and infection and dose optimization of therapeutic antibody. Bioluminescence detection with LUMABS (renamed mNeonG-LUMABS, where mNeonG is short for mNeonGreen) sensors based on bioluminescence resonance energy transfer (BRET) between blue-emitting luciferase Nluc and green fluorescent protein (FP) mNeonGreen has been demonstrated to enable fast detection of antibodies directly in serum with reasonable sensitivity. However, some mNeonG-LUMABS sensors exhibit low sensitivity, and thus, sensitivity improvement remains imperative. Here, we report a bright green FP, Clover4, obtained by structure-guided mutagenesis of green FP Clover. Despite similar brightness and fluorescence spectra of Clover and mNeonGreen, Clover4-LUMABS sensors exhibit a largely increased dynamic range (maximum 20-fold) and much lower limit of detection (LOD) (maximum 5.6-fold), most likely because Clover4 is positioned in a more parallel orientation to Nluc in LUMABS. Due to modular design, Clover4-LUMABS offers a general BRET system for fast and highly sensitive antibody detection in serum.
关键词	Antibody Detection Bret Clover4 Fluorescent Protein In Vitro Diagnostics Luciferase Nluc
相关链接	[Scopus记录]
收录类别	SCI ; EI
语种	英语
重要成果	NI期刊
学校署名	其他
资助项目	National Key Research and Development Program of China["2017YFA0700403","2020YFA0908802"] ; National Natural Science Foundation (NSFC) of China[81927803,21874145,32000732,32000731] ; Natural Science Foundation of Shenzhen["JCYJ20170818164040422","JCYJ20200109115633343"] ; Department of Science and Technology of Guangdong Province["2020B121201010","2020A1515010675"]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000747115200043
出版者	AMER CHEMICAL SOC
EI入藏号	20214711190980
EI主题词	Bioluminescence ; Chemical detection ; Diagnosis ; Energy transfer ; Fluorescence ; Phosphorescence
EI分类号	Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Light/Optics:741.1 ; Chemistry:801
Scopus记录号	2-s2.0-85119264925
来源库	Scopus
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/256932
专题	生命科学学院_生物系生命科学学院
作者单位	1.Guangdong Prov. Key Lab. of Biomed. Optical Imaging Technol. and Ctr. for Biomed. Opt. and Molec. Imaging and Cas Key Lab. of Hlth. Info.,Center for Micro Nano Systems and Bionic Medicine,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China 2.Institute of Systems and Physical Biology,Shenzhen Bay Laboratory,Shenzhen,518132,China 3.Faculty of Health Sciences,University of Macau,Macau SAR,999078,Macao 4.Department of Biology,Southern University of Science and Technology,Shenzhen,518060,China 5.College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,518060,China 6.School of Life and Health Sciences,Kobilka Institute of Innovative Drug Discovery,Chinese University of Hong Kong,Shenzhen,518172,Hong Kong 7.Ministry of Education Key Laboratory for Membrane-less Organelles and Cellular Dynamics,Hefei National Laboratory for Physical Sciences at the Microscale,School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,230027,China 8.State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,Center for Molecular Imaging and Translational Medicine,School of Public Health,Xiamen University,Xiamen,361005,China
推荐引用方式 GB/T 7714	Deng，Mengying,Yuan，Jing,Yang，Haibin,et al. A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein[J]. ACS Nano,2021,15(11):17602-17612.
APA	Deng，Mengying.,Yuan，Jing.,Yang，Haibin.,Wu，Xuli.,Wei，Xiaoyuan.,...&Chu，Jun.(2021).A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein.ACS Nano,15(11),17602-17612.
MLA	Deng，Mengying,et al."A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein".ACS Nano 15.11(2021):17602-17612.