Identification of TRPV4 as a novel target in invasiveness of colorectal cancer

Zhang, Peng1,2; Xu, Jian1,2; Zhang, Hua1,2; Liu, Xiao-Yu3,4

2021-11-23

10.1186/s12885-021-08970-7

BMC CANCER   影响因子和分区

1471-2407

Background Emerging evidence has indicated the critical role of TRPV4 in diverse human cancers. However, the underlying molecular mechanism of TRPV4 in colon cancer invasiveness is still unknown. Methods Immunohistochemistry staining was used to analyze the expression of TRPV4 and ZEB1 in clinical tissues; Wound healing and transwell assays were applied to determine the cell invasiveness; Western blot was used to explore the relation between TRPV4 and ZEB1. Results Colon cancer cells were transfected with siRNA against TRPV4 or HC067047 (a selective TRPV4 antagonist), TRPV4 full-length plasmid or siRNA against ZEB1, or both, in order to measure cell migration and invasion. And we found that TRPV4 silencing or inhibition exhibited an inhibitory role in colon cancer cell migration and invasion, coupled with compromised EMT process, and suppressed AKT activity. TRPV4 stimulated expression of ZEB1 and consequently contributed to EMT process and invasiveness. It was also revealed that overexpression of TRPV4 and ZEB1 in clinical patients with local metastasis, and positive correlation between TRPV4 and ZEB1. Conclusions Our results uncovered the role of TRPV4 in tumor metastasis and highlighted the potential mechanism of TRPV4-ZEB1 axis in indicating EMT.

Colon cancer TRPV4 Invasiveness ZEB1

SCI

英语

通讯

National Natural Science Foundation of China[82003108] ; Natural Science Foundation of Jiangsu Province[BK20190595] ; Fundamental Research Funds for the Central Universities[JUSRP11956] ; Guangdong Basic and Applied Basic Research Foundation[2021A1515010970] ; Shenzhen Key Medical Discipline Construction Fund[SZXK039]

Oncology

Oncology

WOS:000721880600004

BMC

CLINICAL MEDICINE

Web of Science

1.Longgang ENT Hosp, 3004 Longgang Ave, Shenzhen, Guangdong, Peoples R China
2.Inst ENT, Shenzhen Key Lab ENT, 3004 Longgang Ave, Shenzhen, Guangdong, Peoples R China
3.Jiangnan Univ, Sch Med, Wuxi, Jiangsu, Peoples R China
4.Southern Univ Sci & Technol, Sch Med, 1088 Xueyuan Ave, Shenzhen, Guangdong, Peoples R China

Zhang, Peng,Xu, Jian,Zhang, Hua,et al. Identification of TRPV4 as a novel target in invasiveness of colorectal cancer[J]. BMC CANCER,2021,21(1).

Zhang, Peng,Xu, Jian,Zhang, Hua,&Liu, Xiao-Yu.(2021).Identification of TRPV4 as a novel target in invasiveness of colorectal cancer.BMC CANCER,21(1).

Zhang, Peng,et al."Identification of TRPV4 as a novel target in invasiveness of colorectal cancer".BMC CANCER 21.1(2021).