| 题名 | Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation |
| 作者 | |
| 通讯作者 | Sisi,Li; Steven E.,Jacobsen; Jiamu,Du |
| 共同第一作者 | Qian,Wang; Yan,Xue; Laixing,Zhang |
| 发表日期 | 2021-11-26
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| DOI | |
| 发表期刊 | |
| ISSN | 0036-8075
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| EISSN | 1095-9203
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| 卷号 | 374期号:6571页码:1152-1157 |
| 摘要 | In eukaryotes, small RNAs (sRNAs) play critical roles in multiple biological processes. Dicerendonucleases are a central part of sRNA biogenesis. In plants, DICER-LIKE PROTEIN 3 (DCL3) produces 24-nucleotide (nt) small interfering RNAs (siRNAs) that determine the specificity of the RNA-directed DNA methylation pathway. Here, we determined the structure of a DCL3–pre-siRNA complex in an active dicing-competent state. The 5′-phosphorylated A1 of the guide strand and the 1-nt 3′ overhang of the complementary strand are specifically recognized by a positively charged pocket and an aromatic cap, respectively. The 24-nt siRNA length dependence relies on the separation between the 5′-phosphorylated end of the guide RNA and dual cleavage sites formed by the paired ribonuclease III domains. These structural studies, complemented by functional data, provide insight into the dicing principle for Dicers in general. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI论文
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| 学校署名 | 第一
; 通讯
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| 资助项目 | National Key RD Program[2016YFA0503200]
; Shenzhen Science and Technology Program[
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| WOS研究方向 | Science & Technology - Other Topics
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| WOS类目 | Multidisciplinary Sciences
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| WOS记录号 | WOS:000725668600051
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| 出版者 | |
| ESI学科分类 | BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;COMPUTER SCIENCE;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;ENGINEERING
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| 来源库 | 人工提交
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| 引用统计 |
被引频次[WOS]：55
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/257313 |
| 专题 | 南方科技大学-北京大学植物与食品联合研究所 生命科学学院 生命科学学院_生物系 |
| 作者单位 | 1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China. 2.Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA. 3.Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China. 4.Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK. 5.Department of Biochemistry and Molecular Biology, International Cancer Center, Shenzhen University Health Science Center, Shenzhen 518060, China. 6.Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA. |
| 第一作者单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 通讯作者单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 第一作者的第一单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 推荐引用方式 GB/T 7714 |
Qian,Wang,Yan,Xue,Laixing,Zhang,et al. Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation[J]. SCIENCE,2021,374(6571):1152-1157.
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| APA |
Qian,Wang.,Yan,Xue.,Laixing,Zhang.,Zhenhui,Zhong.,Suhua,Feng.,...&Jiamu,Du.(2021).Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation.SCIENCE,374(6571),1152-1157.
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| MLA |
Qian,Wang,et al."Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation".SCIENCE 374.6571(2021):1152-1157.
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| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | 操作 | |
| 2021.Science.pdf（2072KB） | -- | -- | 限制开放 | -- | ||
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