MCM7 affects the cisplatin resistance of liver cancer cells and the development of liver cancer by regulating the PI3K/Akt signaling pathway

Su, Dongna1,2

2021-11-01

10.1080/08923973.2021.1991372

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY   影响因子和分区

0892-3973

1532-2513

Objective Aberrant DNA replication is regarded as a component of cancer development. Minichromosome maintenance protein 7 (MCM7), which is critical for the initiation of DNA replication, is overexpressed in multiple malignancies. The effect of MCM7 on cell proliferation, apoptosis, and drug resistance of liver cancer and its mechanism were investigated in this study. Methods MCM7 expression in normal liver cells, liver cancer cell lines, and tissues, as well as adjacent tissues, was determined by qRT-PCR. CCK-8 and flow cytometry was performed to detect cell viability, apoptosis, and cell cycle, respectively. The related mRNA and protein expressions were detected by qRT-PCR and western blot. Results High expression of MCM7 was found in liver cancer tissues and cells, which results in notably lower survival time of patients. Cisplatin (DDP) could inhibit cell proliferation and affect MCM7 expression. Silencing of MCM7 inhibited cell viability, promoted cell apoptosis, arrested cell cycle at G1 phase, and enhanced the effect of DDP on cancer cells, while overexpression of MCM7 did the opposite. Moreover, silencing of MCM7 inhibited cyclinD1 and Ki-67 expressions. The overexpression of MCM7 increased phosphorylation levels of PI3K and AKT, activated the PI3K/AKT pathway, and weakened the inhibitory effect of DDP on the PI3K/AKT pathway. Conclusion Silencing of MCM7 may inhibit cell proliferation and promote apoptosis by regulating the PI3K/AKT pathway to affect the cell cycle, thus affecting the development of liver cancer, and improving the sensitivity of liver cancer cells to DDP.

Minichromosome maintenance protein 7 liver cancer PI3K AKT drug resistance

SCI

英语

通讯

Immunology ; Pharmacology & Pharmacy ; Toxicology

Immunology ; Pharmacology & Pharmacy ; Toxicology

WOS:000722520200001

TAYLOR & FRANCIS LTD

IMMUNOLOGY

Web of Science

1.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Infect Dis, Guangzhou, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, 1017 DongMen Bei Rd, Shenzhen 518020, Peoples R China

Su, Dongna. MCM7 affects the cisplatin resistance of liver cancer cells and the development of liver cancer by regulating the PI3K/Akt signaling pathway[J]. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY,2021.

Su, Dongna.(2021).MCM7 affects the cisplatin resistance of liver cancer cells and the development of liver cancer by regulating the PI3K/Akt signaling pathway.IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY.

Su, Dongna."MCM7 affects the cisplatin resistance of liver cancer cells and the development of liver cancer by regulating the PI3K/Akt signaling pathway".IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2021).