Complete Freund's adjuvant-induced protein dysregulation correlated with mirror image pain as assessed by quantitative proteomics of the mouse spinal cord

Ma，Quan1,2; Chen，Xi3; Sun，Jinli4; Wang，Li5; Jiang，Weichao3; Zhang，Xi6; Chen，Sifang3

2022-01-22

10.1016/j.bbrc.2021.11.032

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Inflammation or trauma occurring on one side of the body can cause pathological pain on the contralateral noninjured side in a phenomenon called mirror-image pain (MIP). Although some potential mechanisms involved in MIP have been reported, including those involving the immune system and glial cells as well as neural mechanisms, the molecular mechanisms are not well understood. In this study, we aimed to understand the molecular mechanisms in MIP using quantitative proteomics and whole-cell patch clamp recordings. Behavioral test results showed that complete Freund's adjuvant could induce MIP in the mice. The results of isobaric tags for relative and absolute quantification (iTRAQ) quantitative proteomics showed that 108 proteins were dysregulated, and these proteins may represent potential targets. Furthermore, bioinformatics analysis was applied to explore the potential molecular mechanisms during MIP after complete Freund's adjuvant (CFA) treatment. Parallel reaction monitoring (PRM) results showed that PKCδ and seven other dysregulated proteins were related to MIP after CFA treatment. Patch clamp recording results showed that CFA treatment could increase intrinsic excitability and spontaneous firing in spinal cord neurons during MIP. In summary, we found that CFA could induce MIP. The results of proteomic research on the spinal cord after CFA treatment could provide new insight into the molecular mechanisms of MIP. Moreover, the neuronal activity of spinal cord neurons was upregulated during MIP after CFA treatment. In summary, the results of the spinal cord proteomic profile provide a potential molecular mechanism for understanding MIP.

Complete Freund's adjuvant Mirror image pain Patch clamp recording PKCδ Quantitative proteomic

SCI

英语

其他

Natural Science Foundation of Fujian Province, China[2019J01574] ; Natural Science Founda-tion for Youths of Fujian Health and Family Planning Commission, China[2019-2-48] ; Medical and Health Guidance Program of Xiamen City, China[3502Z20214ZD1021]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000729421800004

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

2-s2.0-85120655890

Scopus

1.School of Life Science and Technology,Harbin Institute of Technology,Harbin,150001,China
2.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
3.Department of Neurosurgery,The First Affiliated Hospital of Xiamen University,Xiamen,361003,China
4.Department of Reproduction,The First Affiliated Hospital of Xiamen University,Xiamen,China
5.Office,Luohu District Centers for Disease Control and Prevention,Shenzhen,518000,China
6.Department of Gastroenterology,The First Affiliated Hospital of Shenzhen University,Shenzhen People's Second Hospital,Shenzhen,518000,China

Ma，Quan,Chen，Xi,Sun，Jinli,et al. Complete Freund's adjuvant-induced protein dysregulation correlated with mirror image pain as assessed by quantitative proteomics of the mouse spinal cord[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,589:23-28.

Ma，Quan.,Chen，Xi.,Sun，Jinli.,Wang，Li.,Jiang，Weichao.,...&Chen，Sifang.(2022).Complete Freund's adjuvant-induced protein dysregulation correlated with mirror image pain as assessed by quantitative proteomics of the mouse spinal cord.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,589,23-28.

Ma，Quan,et al."Complete Freund's adjuvant-induced protein dysregulation correlated with mirror image pain as assessed by quantitative proteomics of the mouse spinal cord".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 589(2022):23-28.