Polarized structured illumination microscopy

Xi，Peng1,2; Zhanghao，Karl1,2; Li，Meiqi1

10.1117/12.2615382

2021

Biomedical Imaging and Sensing Conference

0277-786X

1996-756X

978-1-5106-4719-0

Proceedings of SPIE - The International Society for Optical Engineering

11925

2021

null,null,ELECTR NETWORK

1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

SPIE-INT SOC OPTICAL ENGINEERING

Fluorescence polarization microscopy plays a vital role in studying molecular structures and dynamics of biocomplexes by imaging both the intensity and orientation of fluorescent dipoles. Super-resolution (SR) microscopy provides the ultrastructure of subcellular organelles, yet conventional SR lacks the dipole orientation information. We developed polarized structured illumination microscopy (pSIM) to achieve fluorescence super-resolution polarization microscopy.

Fluorescent dipole Polarization modulation Structured illumination microscopy Super-resolution

其他

英语

CPCI-S ; EI

Engineering ; Optics ; Imaging Science & Photographic Technology

Engineering, Biomedical ; Optics ; Imaging Science & Photographic Technology

WOS:000792682600001

20214911279802

Molecular imaging ; Molecular orientation ; Optical resolving power ; Polarization

Biomedical Engineering:461.1 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Mechanics:931.1 ; Electronic Structure of Solids:933.3

2-s2.0-85120449431

Scopus

1.Department of Biomedical Engineering,College of Future Technology Peking University,Beijing,China
2.UTS-SUStech Joint Research Centre for Biomedical Materials and Devices,Department of Biomedical Engineering,College of Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,China

Xi，Peng,Zhanghao，Karl,Li，Meiqi. Polarized structured illumination microscopy[C]. 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA:SPIE-INT SOC OPTICAL ENGINEERING,2021.