Light-sheet microscopy in the near-infrared II window

Wang, Feifei1; Wan, Hao1; Ma, Zhuoran1; Zhong, Yeteng1; Sun, Qinchao1; Tian, Ye1; Qu, Liangqiong2,3; Du, Haotian1; Zhang, Mingxi4; Li, Lulin5; Ma, Huilong6; Luo, Jian5,7; Liang, Yongye6; Li, Wen Jung8,9; Hong, Guosong10; Liu, Lianqing8; Dai, Hongjie1

2019-06

10.1038/s41592-019-0398-7

NATURE METHODS   影响因子和分区

1548-7091

1548-7105

Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.

SCI

英语

NI期刊

其他

National Institutes of Health[DP1-NS-105737]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000469455200024

NATURE PUBLISHING GROUP

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Stanford Univ, Dept Chem & BioX, Stanford, CA 94305 USA
2.Univ N Carolina, Dept Radiol, Chapel Hill, NC USA
3.Univ N Carolina, BRIC, Chapel Hill, NC USA
4.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan, Hubei, Peoples R China
5.VA Palo Alto Hlth Care Syst, Palo Alto Vet Inst Res, Palo Alto, CA USA
6.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen, Peoples R China
7.Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA
8.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang, Liaoning, Peoples R China
9.City Univ Hong Kong, Dept Mech Engn, Hong Kong, Peoples R China
10.Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA

Wang, Feifei,Wan, Hao,Ma, Zhuoran,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-+.

Wang, Feifei.,Wan, Hao.,Ma, Zhuoran.,Zhong, Yeteng.,Sun, Qinchao.,...&Dai, Hongjie.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-+.

Wang, Feifei,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-+.