南方科技大学知识苑(SUSTech KC): Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

题名	Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
作者	Chang, Xiaoyan 1; Zhou, Keru 1; Liu, Yanguang 1,2; Duan, Lian 3; Zhang, Lichun 2; Zhang, Guoliang 3; Wang, Haikun 4; Wang, Guiqin 1
通讯作者	Zhang, Guoliang; Wang, Haikun; Wang, Guiqin
发表日期	2021-11-01
DOI	10.3791/62626
发表期刊	Jove-Journal of Visualized Experiments 影响因子和分区
ISSN	1940-087X
期号	177
摘要	Since 1996, A/goose/Guangdong/1/96-lineage highly pathogenic avian influenza (HPAI) H5 viruses have been causing flu outbreaks in poultry and wild birds. Occasionally, humans also fall victim to it, which results in high mortality. Nonetheless, HPAI virus research is often hindered, considering that it must be handled within biosafety level 3 laboratories. To address this issue, pseudoviruses are adopted as an alternative to wild-type viruses in some experiments of H5 HPAI studies. Pseudoviruses prove to be the ideal tools to study neutralizing antibodies against H5 HPAI viruses. This protocol describes the procedures and critical steps of H5 HPAI pseudovirus preparations and pseudovirus neutralization assays. Also, it discusses the troubleshooting, limitation, and modifications of these assays.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Innovation Capacity Building Project of Jiangsu province[BM2020019] ; Shenzhen Scientific and Technological Project[JSGG20200225150702770] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29030103] ; Guangdong Scientific and Technological Project[2020B1111340076] ; Shenzhen Bay Laboratory Open Research Program[SZBL202002271003]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000726748200014
出版者	JOURNAL OF VISUALIZED EXPERIMENTS
来源库	Web of Science
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/258533
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Nanjing Adv Acad Life & Hlth, Nanjing, Peoples R China 2.Jilin Acad Agr Sci, Inst Anim Biotechnol, Changchun, Peoples R China 3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China 4.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Pasteur Shanghai, CAS Key Lab Mol Virol & Immunol, Beijing, Peoples R China
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Chang, Xiaoyan,Zhou, Keru,Liu, Yanguang,et al. Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity[J]. Jove-Journal of Visualized Experiments,2021(177).
APA	Chang, Xiaoyan.,Zhou, Keru.,Liu, Yanguang.,Duan, Lian.,Zhang, Lichun.,...&Wang, Guiqin.(2021).Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity.Jove-Journal of Visualized Experiments(177).
MLA	Chang, Xiaoyan,et al."Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity".Jove-Journal of Visualized Experiments .177(2021).