Lipid Droplet-Specific Probe for Rapidly Locating Atherosclerotic Plaques and Intraoperative Imaging via in Situ Spraying

Sang，Mangmang1; Cai，Bicheng2; Qin，Shuheng3; Zhao，Shanshan1; Mao，Yijie1; Wang，Yan1; Yu，Xiu2; Zheng，Jinrong1

2021

10.1021/acsami.1c17797

ACS Applied Materials & Interfaces   影响因子和分区

1944-8244

1944-8252

The ability to visualize the full extent of atherosclerotic plaques during surgery has major implications for therapeutic outcomes. Fluorescence imaging is a promising approach for atherosclerotic plaque inspection during surgery. However, a specific strategy for the intraoperative fluorescence imaging of atherosclerosis has not been established. This study presents an in situ spraying aerosol of a lipid droplet-specific probe to rapidly and precisely locate atherosclerotic plaques during surgery. Stable imaging of the plaque was achieved within 5 min by nebulizing the aqueous solution of the lipid droplet-specific probe (CN-PD) into 3 μm droplets and rapidly permeating it in situ. The visible fluorescence bioimaging of CN-PD can accurately delineate the plaque margins and size even with a diameter ≤0.5 mm, which are capable of being swiftly captured during the stable plaque imaging window (>2 h). This strategy combines the consideration of a specific probe design and an efficient in situ delivery, which results in weak interference from the background signals. Therefore, the plaque-to-normal tissue ratio (P/N) is sufficient to facilitate the surgical delineation of carotid atherosclerotic plaques. The originality of the intraoperative fluorescence imaging of the plaques via in situ delivery of the lipid droplet-specific probe holds promise for effective clinical application.

atherosclerosis fluorescence imaging foam cells imaging-guided surgery lipid droplets

SCI ; EI

英语

通讯

Xiamen medical and health guidance project[3502Z20209144] ; National Natural Science Foundation of China[81770028,82100443]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000730567900001

AMER CHEMICAL SOC

20215011329770

Diseases ; Drops ; Probes ; Surgery

Medicine and Pharmacology:461.6 ; Imaging Techniques:746

2-s2.0-85121053800

Scopus

1.Institute of Cardiovascular Diseases,Xiamen Cardiovascular Hospital,Xiamen University,Xiamen,2999 Jinshan Road, Huli District,361006,China
2.Shenzhen Key Laboratory of Respiratory Diseases,Shenzhen People's Hospital,Southern University of Science and Technology,Shenzhen,3046 Shennan East Road, Luohu District,518055,China
3.Department of Biomedical Engineering,School of Engineering,China Pharmaceutical University,Nanjing,24 Tongjia Lane, Gulou District,210009,China

Sang，Mangmang,Cai，Bicheng,Qin，Shuheng,et al. Lipid Droplet-Specific Probe for Rapidly Locating Atherosclerotic Plaques and Intraoperative Imaging via in Situ Spraying[J]. ACS Applied Materials & Interfaces,2021,13(49):58369-58381.

Sang，Mangmang.,Cai，Bicheng.,Qin，Shuheng.,Zhao，Shanshan.,Mao，Yijie.,...&Zheng，Jinrong.(2021).Lipid Droplet-Specific Probe for Rapidly Locating Atherosclerotic Plaques and Intraoperative Imaging via in Situ Spraying.ACS Applied Materials & Interfaces,13(49),58369-58381.

Sang，Mangmang,et al."Lipid Droplet-Specific Probe for Rapidly Locating Atherosclerotic Plaques and Intraoperative Imaging via in Situ Spraying".ACS Applied Materials & Interfaces 13.49(2021):58369-58381.