Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment

Li Sijing1,2; Ma Fenbo2; Pang Xiangchao2,5; Tang Bin2,3,4; Lin Lijun1

2019-05-15

10.1016/j.carbpol.2019.02.061

CARBOHYDRATE POLYMERS   影响因子和分区

0144-8617

1879-1344

Magnesium chondroitin sulfate (MgCS) has been fabricated and characterized in this study. We investigated its morphology, composition as well as structure. The results verify that the sodium of sodium chondroitin sulfate (CS) is successfully replaced by magnesium and formed a polysaccharide-metal ion complex. To evaluate the clinical potential of MgCS, cell proliferation and apoptosis test were conducted. The results reveal that MgCS could effectively increase the proliferation and decrease the apoptosis of osteoarthritis (OA) chondrocytes. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate the gene expression level. RT-qPCR analysis suggests that MgCS could significantly increase the expression of COLII and decrease the expression of IL-1 beta and iNOS in OA chondrocytes. Furthermore, significant upregulation of Bcl-2 mRNA expression and downregulation of the expression of apoptosis related gene p53 were observed. Thus, it is indicated that MgCS should have great potentials in OA treatment.

Chondroitin sulfate Magnesium Anti-inflammatory Apoptosis

SCI ; EI

英语

通讯

Guangzhou Scientific and Technique Department[201704020129] ; Guangzhou Scientific and Technique Department[201704020069]

Chemistry ; Polymer Science

Chemistry, Applied ; Chemistry, Organic ; Polymer Science

WOS:000459981900043

ELSEVIER SCI LTD

20190906552202

Cell death ; Cell proliferation ; Gene expression ; Magnesium ; Metal ions ; Metals ; Polymerase chain reaction ; Sodium compounds ; Sulfur compounds

Biology:461.9 ; Metallurgy:531.1 ; Magnesium and Alloys:542.2 ; Biochemistry:801.2

CHEMISTRY

Web of Science

1.Southern Med Univ, Zhujiang Hosp, Dept Orthoped, Guangzhou, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China
3.Guangdong Prov Key Lab Cell Microenvironm & Dis R, Guangzhou, Guangdong, Peoples R China
4.Shenzhen Key Lab Cell Microenvironm, Shenzhen, Peoples R China
5.Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha, Hunan, Peoples R China

Li Sijing,Ma Fenbo,Pang Xiangchao,et al. Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment[J]. CARBOHYDRATE POLYMERS,2019,212:387-394.

Li Sijing,Ma Fenbo,Pang Xiangchao,Tang Bin,&Lin Lijun.(2019).Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment.CARBOHYDRATE POLYMERS,212,387-394.

Li Sijing,et al."Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment".CARBOHYDRATE POLYMERS 212(2019):387-394.