Programmable Electrodeposition of Janus Alginate/Poly-L-Lysine/Alginate (APA) Microcapsules for High-Resolution Cell Patterning and Compartmentalization

Liu, Zeyang1,2; Nan, Haochen1; Jiang, Yike2; Xu, Tao2; Gong, Xiaohua3,4; Hu, Chengzhi1,5

2021-12-01

10.1002/smll.202106363

Small   影响因子和分区

1613-6810

1613-6829

Encapsulation of live cells in protective, semipermeable microcapsules is one of the kernel techniques for in vitro tissue regeneration, cell therapies, and pharmaceutical screening. Advanced fabrication techniques for cell encapsulation have been developed to meet different requirements. Existing cell encapsulation techniques place substantial constraints on the spatial patterning of live cells as well as on the compartmentalization of heterotypic cells. Alginate-Poly-L-lysine-alginate (APA) microcapsules that use sodium alginate as the polyanion and poly-L-lysine (PLL) as the polycation have been extensively employed for cell microencapsulation due to their excellent biocompatibility and biodegradability. This study proposes a novel method for developing programmable Janus APA microcapsules with variable shapes and sizes by using electrodeposition. By the versatile design of the microelectrode device, sequential electrodeposition is triggered to electro-address the cells at specific locations immobilized within a Janus APA microcapsule. The osteogenesis is evaluated by resembling cell compartmentalized and vascularized osteoblast-laden constructs. This technique allows precise spatial patterning of heterotypic cells inside the APA microcapsule, enabling the observation of cellular growth, interactions, and differentiation in a well-controlled chemical and mechanical microenvironment.

cell compartmentalization cell patterning electrodeposition Janus alginate-Poly-L-lysine-alginate (APA) microcapsules

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[61903177] ; Shenzhen Science and Technology Program[JCYJ20190809144013494] ; Science and Technology Program of Guangdong[2021A1515011813] ; Science, Technology, and Innovation Commission of Shenzhen Municipality[ZDSYS20200811143601004]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000731316300001

WILEY-V C H VERLAG GMBH

20215111370558

Amino acids ; Biodegradability ; Microelectrodes ; Microstructure ; Sodium alginate ; Tissue regeneration

Biomedical Engineering:461.1 ; Immunology:461.9.1 ; Biochemistry:801.2 ; Organic Compounds:804.1 ; Materials Science:951

Web of Science

1.Southern Univ Sci & Technol, Dept Mech & Energy Engn, Shenzhen Key Lab Biomimet Robot & Intelligent Sys, Shenzhen 518055, Peoples R China
2.Tsinghua Berkeley Shenzhen Inst TBSI, Stem Cell Therapy & Regenerat Med Lab, 1001 Xueyuan Ave, Shenzhen 518000, Peoples R China
3.Univ Calif Berkeley, Sch Optometry, 380 Minor Ln, San Francisco, CA 94720 USA
4.Univ Calif Berkeley, Vis Sci Program, 380 Minor Ln, San Francisco, CA 94720 USA
5.Southern Univ Sci & Technol, Guangdong Prov Key Lab Human Augmentat & Rehabil, Shenzhen 518055, Peoples R China

Liu, Zeyang,Nan, Haochen,Jiang, Yike,et al. Programmable Electrodeposition of Janus Alginate/Poly-L-Lysine/Alginate (APA) Microcapsules for High-Resolution Cell Patterning and Compartmentalization[J]. Small,2021,18.

Liu, Zeyang,Nan, Haochen,Jiang, Yike,Xu, Tao,Gong, Xiaohua,&Hu, Chengzhi.(2021).Programmable Electrodeposition of Janus Alginate/Poly-L-Lysine/Alginate (APA) Microcapsules for High-Resolution Cell Patterning and Compartmentalization.Small,18.

Liu, Zeyang,et al."Programmable Electrodeposition of Janus Alginate/Poly-L-Lysine/Alginate (APA) Microcapsules for High-Resolution Cell Patterning and Compartmentalization".Small 18(2021).